• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

GABRA4 Recombinant antibody

GABRA4 Recombinant Antibody for FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC, ELISA

Conjugate

Unconjugated

CloneNo.

230302A4

Cat No : 83030-4-RR

Print datasheet

Synonyms

GABRA4

验证数据展示

  • FC experiment of SH-SY5Y using 83030-4-RR

    FC experiment of SH-SY5Y using 83030-4-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Anti-Human GABRA4 (83030-4-RR, Clone:230302A4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of PC-3 using 83030-4-RR

    FC experiment of PC-3 using 83030-4-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug Anti-Human GABRA4 (83030-4-RR, Clone:230302A4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inPC-3 cells, SH-SY5Y cells

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83030-4-RR targets GABRA4 in FC, ELISA applications and shows reactivity with Human samples.

Tested Applications FC, ELISA
Tested Reactivity Human
Immunogen GABRA4 fusion protein Ag3600 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name gamma-aminobutyric acid (GABA) A receptor, alpha 4
Synonyms GABRA4
Calculated Molecular Weight 554 aa, 62 kDa
Observed Molecular Weight 62-70 kDa
GenBank Accession NumberBC035055
Gene Symbol GABRA4
Gene ID (NCBI) 2557
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP48169
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. GABRA4, encoding the extrasynaptically located α4‐subunit of the GABAA receptor, is involved in the etiology of autism. (PMID: 32028044, 32033586)

实验方案

Product Specific Protocols
FC protocol for GABRA4 antibody 83030-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols