验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, A375 cells, HEK-293 cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| IP | See 1 publications below |
产品信息
20870-1-AP targets GABPB2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | GABPB2 fusion protein Ag14933 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | GA binding protein transcription factor, beta subunit 2 |
| 别名 | GABPB-2, GABPB 2, GABP subunit beta-2, GABP subunit beta 2, GA-binding protein subunit beta-2 |
| 计算分子量 | 448 aa, 49 kDa |
| 观测分子量 | 49 kDa |
| GenBank蛋白编号 | BC027033 |
| 基因名称 | GABPB2 |
| Gene ID (NCBI) | 126626 |
| RRID | AB_10734321 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TAK5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GABPB2 is a subunit of the GA-binding protein (GABP) transcription factor, which is a heterodimeric complex. The GABP complex plays a role in the regulation of gene transcription by binding to specific DNA sequences. GABPB2 specifically contributes to the transactivation of target genes (PMID: 18628204). The protein is primarily located in the nucleus. It is ubiquitously expressed in various tissues, including testis, ovary, and others. Studies have shown that GABPB2 is dispensable for normal lymphocyte development but moderately affects B cell responses (PMID: 18628204). Mice deficient in GABPB2 exhibit increased B cell proliferation and antibody production in response to certain stimuli. The molecular weight of GABPB2 is 48 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GABPB2 antibody 20870-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
