验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27567-1-AP targets GABBR2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | Human, Mouse, Rat |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 |
CatNo: Ag25886 Product name: Recombinant human GABBR2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 685-883 aa of BC035071 Sequence: KLITLRTNPDAATQNRRFQFTQNQKKEDSKTSTSVTSVNQASTSRLEGLQSENHHLRMKITELDKDLEEVTMQLQDTPEKTTYIKQNHYQELNDILNLGNFTESTDGGKAILKNHLDQNPQLQWNTTEPSRTCKDPIEDINSPEHIQRRLSLQLPILHHAYLPSIGGVDASCVSPCVSPTASPRHRHVPPSFRVMVSGL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | gamma-aminobutyric acid (GABA) B receptor, 2 |
| 别名 | G protein coupled receptor 51, GABA B R2, GABA B receptor 2, GABA BR2, GABABR2, GABBR2, Gb2, GPR51, GPRC3B, HG20, HRIHFB2099 |
| 计算分子量 | 941 aa, 106 kDa |
| 观测分子量 | 120 kDa |
| GenBank蛋白编号 | BC035071 |
| 基因名称 | GABBR2 |
| Gene ID (NCBI) | 9568 |
| RRID | AB_2880911 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75899 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for GABBR2 antibody 27567-1-AP | Download protocol |
| WB protocol for GABBR2 antibody 27567-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Cancer-cell-derived GABA promotes β-catenin-mediated tumour growth and immunosuppression. | ||
World J Gastroenterol γ-aminobutyric acid B2 receptor: A potential therapeutic target for cholangiocarcinoma in patients with diabetes mellitus | ||
Biochem Biophys Res Commun GAD1-mediated GABA elicits aggressive characteristics of human oral cancer cells | ||
Life Sci GABA regulates the proliferation and apoptosis of head and neck squamous cell carcinoma cells by promoting the expression of CCND2 and BCL2L1 | ||
Neurorehabil Neural Repair Metabolomics-Based Effects of a Natural Product on Remyelination After Cerebral Ischemia Injury Via GABABR-pCREB-BDNF Pathway | ||
Behav Brain Res Impact of Abl2/Arg Deficiency on Anxiety and Depressive Behaviors in mice |
