验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, 37°C incubated mouse brain tissue, rat brain tissue, 37°C incubated rat brain tissue |
| Positive IHC detected in | mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
13940-1-AP targets GABA B receptor 1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag4838 Product name: Recombinant human GABBR1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 101-471 aa of BC041332 Sequence: SKCDPGQATKYLYELLYNDPIKIILMPGCSSVSTLVAEAARMWNLIVLSYGSSSPALSNRQRFPTFFRTHPSATLHNPTRVKLFEKWGWKKIATIQQTTEVFTSTLDDLEERVKEAGIEITFRQSFFSDPAVPVKNLKRQDARIIVGLFYETEARKVFCEVYKERLFGKKYVWFLIGWYADNWFKIYDPSINCTVDEMTEAVEGHITTEIVMLNPANTRSISNMTSQEFVEKLTKRLKRHPEETGGFQEAPLAYDAIWALALALNKTSGGGGRSGVRLEDFNYNNQTITDQIYRAMNSSSFEGVSGHVVFDASGSRMAWTLIEQLQGGSYKKIGYYDSTKDDLSWSKTDKWIGGSPPADQTLVIKTFRFLS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | gamma-aminobutyric acid (GABA) B receptor, 1 |
| 别名 | GABAB receptor 1, GABBR1, GABA B R1, GABA BR1, GABA-B receptor 1 |
| 计算分子量 | 961 aa, 108 kDa |
| 观测分子量 | 105-130 kDa |
| GenBank蛋白编号 | BC041332 |
| 基因名称 | GABBR1 |
| Gene ID (NCBI) | 2550 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UBS5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GABA B receptor 1 (GABBR1) is an essential protein subunit that forms the functional GABA B receptor, a metabotropic receptor for the primary inhibitory neurotransmitter in the central nervous system, gamma-aminobutyric acid (GABA). Unlike the fast-acting ionotropic GABA A receptors, GABA B receptors mediate slow, prolonged inhibitory signaling through G-proteins. Two bands with the apparent molecular weights of 130 and 105 kDa were corresponding to GABAB1a and GABAB1b proteins, were identified in the brain extract and membrane extract of wild-type mice (PMID: 12970075).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for GABA B receptor 1 antibody 13940-1-AP | Download protocol |
| WB protocol for GABA B receptor 1 antibody 13940-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
