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验证数据展示

  • WB analysis of COLO 320 using 18724-1-AP (same clone as 18724-1-PBS)

    WB analysis of COLO 320 using 18724-1-AP (same clone as 18724-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18724-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 18724-1-AP (same clone as 18724-1-PBS)

    IHC staining of human heart using 18724-1-AP (same clone as 18724-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18724-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 18724-1-AP (same clone as 18724-1-PBS)

    IHC staining of human heart using 18724-1-AP (same clone as 18724-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18724-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 18724-1-AP (same clone as 18724-1-PBS)

    FC experiment of HepG2 using 18724-1-AP (same clone as 18724-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug GABARAPL2-Specific Polyclonal antibody (18724-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 18724-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 18724-1-AP (same clone as 18724-1-PBS)

    IF Staining of HepG2 using 18724-1-AP (same clone as 18724-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using GABARAPL2-Specific antibody (18724-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 18724-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GABARAPL2-Specific Polyclonal antibody, PBS Only

GABARAPL2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 18724-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

GABARAPL2, ATG8, FLC3A, GABA(A) receptor-associated protein-like 2, Gamma-aminobutyric acid receptor-associated protein-like 2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

18724-1-PBS targets GABARAPL2-Specific in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GABA(A) receptor-associated protein-like 2
别名 GABARAPL2, ATG8, FLC3A, GABA(A) receptor-associated protein-like 2, Gamma-aminobutyric acid receptor-associated protein-like 2
计算分子量 14 kDa
观测分子量 14 kDa
GenBank蛋白编号BC005985
基因名称 GABARAPL2
Gene ID (NCBI) 11345
RRIDAB_2232116
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP60520
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GABARAPL2 (also known as Golgi-associated ATPase enhancer of 16 kDa; GATE-16) was originally characterized as a factor essential for intra-Golgi protein transport. Later GABARAPL2 along with LC3 and GABARAP, were identified as the homologs of yeast ATG8 which is a unique ubiquitin-like protein essential for autophagy. When induced GABARAPL2 is first digested by ATG4 family enzymes to become its mature form: GABARAPL2-I, later conjugated to PE lipid as lipidated form: GABARAPL2-II. The lipidated form migrates faster than its mature form on SDS-PAGE gel. GABARAPL2 is strongly expressed in the brain. This antibody is specific to GABARAPL2. It has no cross-reaction to other GABARAP protein.

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