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验证数据展示

  • WB analysis using 29993-1-AP (same clone as 29993-1-PBS)

    WB analysis using 29993-1-AP (same clone as 29993-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29993-1-AP (GAA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29993-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 29993-1-AP (same clone as 29993-1-PBS)

    IHC staining of mouse liver using 29993-1-AP (same clone as 29993-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29993-1-AP (GAA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29993-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GAA antibody included

GAA Polyclonal antibody, PBS Only

GAA Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29993-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GAA Ready-To-Use IHC Kit. GAA primary antibody included.

产品信息

29993-1-PBS targets GAA in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag28970

Product name: Recombinant human GAA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 38-230 aa of BC040431

Sequence: VPRELSGSSPVLEETHPAHQQGASRPGPRDAQAHPGRPRAVPTQCDVPPNSRFDCAPDKAITQEQCEARGCCYIPAKQGLQGAQMGQPWCFFPPSYPSYKLENLSSSEMGYTATLTRTTPTFFPKDILTLRLDVMMETENRLHFTIKDPANRRYEVPLETPHVHSRAPSPLYSVEFSEEPFGVIVRRQLDGRV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glucosidase, alpha; acid
别名
计算分子量 105 kDa
观测分子量110 kDa
GenBank蛋白编号BC040431
基因名称 GAA
Gene ID (NCBI) 2548
RRIDAB_2923627
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP10253
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GAA (Alpha-1,4-glucosidase) is a lysosomal enzyme involved in the degradation of glycogen within cellular vacuoles. After translation, GAA undergoes proteolytic processing to form two lengths of lysosomal α-glucosidase, and both N-terminal and C-terminal processing occur. Typically, GAA is synthesized as an immature glycoprotein precursor (110 kDa) in the endoplasmic reticulum and undergoes a seriesof proteolytic and N-glycan processing events to yield an intermediate (95 kDa) and two lysosomal (76 and 70 kDa) isoforms. This antibody can detect the 110 kDa precursor form.

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