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验证数据展示

  • WB analysis using 86240-1-RR

    WB analysis using 86240-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86240-1-RR (G6PD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 86240-1-RR

    WB analysis using 86240-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86240-1-RR (G6PD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HT-29 using 86240-1-RR

    WB analysis of HT-29 using 86240-1-RR

    HT-29 cells were subjected to SDS PAGE followed by western blot with 86240-1-RR (G6PD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 86240-1-RR

    IF Staining of HeLa using 86240-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using G6PD antibody (86240-1-RR, Clone: 250831F8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of NIH/3T3 using 86240-1-RR

    IF Staining of NIH/3T3 using 86240-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using G6PD antibody (86240-1-RR, Clone: 250831F8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 86240-1-RR

    Affinity and Kinetic Characterization of 86240-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86240-1-RR against Human G6PD were performed. The affinity constant is below 1 pM.

G6PD Recombinant monoclonal antibody

G6PD Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86240-1-RR

产品说明书

CloneNo. 250831F8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

EC:1.1.1.49, G6P, G6PD1, Glucose-6-phosphate 1-dehydrogenase

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, HT-29 cells, HEK-293 cells, A549 cells
Positive IF/ICC detected inHeLa cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy G6PD Ready-To-Use IHC Kit. G6PD primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86240-1-RR targets G6PD in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag21862

Product name: Recombinant human G6PD protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-515 aa of BC000337

Sequence: MAEQVALSRTQVCGILREELFQGDAFHQSDTHIFIIMGASGDLAKKKIYPTIWWLFRDGLLPENTFIVGYARSRLTVADIRKQSEPFFKATPEEKLKLEDFFARNSYVAGQYDDAASYQRLNSHMNALHLGSQANRLFYLALPPTVYEAVTKNIHESCMSQIGWNRIIVEKPFGRDLQSSDRLSNHISSLFREDQIYRIDHYLGKEMVQNLMVLRFANRIFGPIWNRDNIACVILTFKEPFGTEGRGGYFDEFGIIRDVMQNHLLQMLCLVAMEKPASTNSDDVRDEKVKVLKCISEVQANNVVLGQYVGNPDGEGEATKGYLDDPTVPRGSTTATFAAVVLYVENERWDGVPFILRCGKALNERKAEVRLQFHDVAGDIFHQQCKRNELVIRVQPNEAVYTKMMTKKPGMFFNPEESELDLTYGNRYKNVKLPDAYERLILDVFCGSQMHFVRSDELREAWRIFTPLLHQIELEKPKPIPYIYGSRGPTEADELMKRVGFQYEGTYKWVNPHKL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glucose-6-phosphate dehydrogenase
别名 EC:1.1.1.49, G6P, G6PD1, Glucose-6-phosphate 1-dehydrogenase
计算分子量 59 kDa
观测分子量 60 kDa
GenBank蛋白编号BC000337
基因名称 G6PD
Gene ID (NCBI) 2539
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP11413
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

G6PD(Glucose-6-phosphate 1-dehydrogenase) belongs to the glucose-6-phosphate dehydrogenase family. It is a housekeeping enzyme encoded in mammals by an X-linked gene. G6PD has important functions in intermediary metabolism because it catalyzes the first step in the pentose phosphate pathway and provides reductive potential in the form of NADPH.(PMID:7489710). This protein has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for G6PD antibody 86240-1-RRDownload protocol
WB protocol for G6PD antibody 86240-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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