G3BP2 Recombinant antibody
G3BP2 Recombinant Antibody for FC, IF/ICC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
FC, IF/ICC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
230275D6
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, A549 cells, Jurkat cells, K-562 cells |
| Positive IF detected in | sodium arsenite treated HeLa cells |
| Positive FC detected in | U2OS cells |
Planning an IHC experiment? We recommend our IHCeasy G3BP2 Ready-To-Use IHC Kit. G3BP2 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF) | IF : 1:500-1:2000 |
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82080-4-RR targets G3BP2 in WB, IF, FC, ELISA applications and shows reactivity with Human samples.
| Tested Applications | FC, IF/ICC, WB, ELISA |
| Tested Reactivity | Human |
| Immunogen | G3BP2 fusion protein Ag9355 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | GTPase activating protein (SH3 domain) binding protein 2 |
| Synonyms | G3BP 2, G3BP2, KIAA0660 |
| Calculated Molecular Weight | 482aa,54 kDa; 449aa,51 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | BC011731 |
| Gene Symbol | G3BP2 |
| Gene ID (NCBI) | 9908 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UN86 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (1). The Ras-GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation. They function to protect RNAs from harmful conditions. G3BP2 is mainly distributed in the cytoplasm and participates in the formation of stress granules, cell differentiation, proliferation, and signal transduction. Accumulating evidence has demonstrated that aberrant expression of G3BP2 contributes to cancer initiation and progression, such as high expression of G3BP2 increasing cell stemness, metastasis and chemoresistance in breast cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for G3BP2 antibody 82080-4-RR | Download protocol |
| IF protocol for G3BP2 antibody 82080-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
