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验证数据展示

  • WB analysis using 21519-1-AP (same clone as 21519-1-PBS)

    WB analysis using 21519-1-AP (same clone as 21519-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21519-1-AP (Frizzled 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21519-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 21519-1-AP (same clone as 21519-1-PBS)

    IF Staining of HepG2 using 21519-1-AP (same clone as 21519-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Frizzled 5 antibody (21519-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21519-1-PBS in a different storage buffer formulation.

  • WB analysis using 21519-1-AP (same clone as 21519-1-PBS)

    WB analysis using 21519-1-AP (same clone as 21519-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21519-1-AP (Frizzled 5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21519-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 21519-1-AP (same clone as 21519-1-PBS)

    IHC staining of human stomach cancer using 21519-1-AP (same clone as 21519-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21519-1-AP (Frizzled 5 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21519-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 21519-1-AP (same clone as 21519-1-PBS)

    IF Staining of HepG2 using 21519-1-AP (same clone as 21519-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Frizzled 5 antibody (21519-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21519-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Frizzled 5 Polyclonal antibody, PBS Only

Frizzled 5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21519-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

FZD5, FzE5, Fz-5, Fz 5, Frizzled-5

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

21519-1-PBS targets Frizzled 5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag16082

Product name: Recombinant human FZD5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 524-585 aa of BC114346

Sequence: GKTVESWRRFTSRCCCRPRRGHKSGGAMAAGDYPEASAALTGRTGPPGPAATYHKQVSLSHV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 frizzled homolog 5 (Drosophila)
别名 FZD5, FzE5, Fz-5, Fz 5, Frizzled-5
计算分子量 585 aa, 65 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC114346
基因名称 FZD5
Gene ID (NCBI) 7855
RRIDAB_2878877
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13467
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fzd5, is the receptor for the Wnt5A ligand. Fzd5 modulates cell fate and cell behavior during vertebrate development. It regulates hematopoiesis by the antagonism of the canonical Wnt pathway, resulting in a pool of quiescent hematopoietic stem cells, and regulates hematopoietic stem cell function in a paracrine fashion through several distinct mechanisms including modulating canonical Wnt signaling. It acts through noncanonical Wnt signaling to promote angiogenesis. It is also involved in control cell orientation, polarity, and directional movement in response to positional cues from chemokine gradients.

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