×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 85829-2-RR

    WB analysis using 85829-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85829-2-RR (Frizzled 10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of Caco-2 using 85829-2-RR

    IF Staining of Caco-2 using 85829-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Frizzled 10 antibody (85829-2-RR, Clone: 243131B5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 85829-2-RR

    Affinity of 85829-2-RR

    Biolayer interferometry (BLI) kinetic assays of 85829-2-RR against Human Frizzled 10 were performed. The affinity constant is 5.91 nM.

Frizzled 10 Recombinant monoclonal antibody

Frizzled 10 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85829-2-RR

产品说明书

CloneNo. 243131B5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

FZD10, CD350, Frizzled-10, FZ 10, Fz-10

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse kidney tissue, rat kidney tissue
Positive IF/ICC detected inCaco-2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85829-2-RR targets Frizzled 10 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12867

Product name: Recombinant human FZD10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 23-229 aa of BC074998

Sequence: SMDMERPGDGKCQPIEIPMCKDIGYNMTRMPNLMGHENQREAAIQLHEFAPLVEYGCHGHLRFFLCSLYAPMCTEQVSTPIPACRVMCEQARLKCSPIMEQFNFKWPDSLDCRKLPNKNDPNYLCMEAPNNGSDEPTRGSGLFPPLFRPQRPHSAQEHPLKDGGPGRGGCDNPGKFHHVEKSASCAPLCTPGVDVYWSREDKRFAVV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 frizzled homolog 10 (Drosophila)
别名 FZD10, CD350, Frizzled-10, FZ 10, Fz-10
计算分子量 581 aa, 65 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC074998
基因名称 Frizzled 10
Gene ID (NCBI) 11211
RRIDAB_3744405
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9ULW2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FZD10 (Frizzled homolog 10) is a member of the frizzled gene family, and considered as a seven-transmembrane Wnt-signaling receptor. FZD10 is expressed at high levels on the cell surface of almost all synovial sarcoma tissues but absent in most normal organs except for placenta. Up-regulation of FZD10 mRNA in several types of human cells might lead to carcinog

实验方案

Product Specific Protocols
IF protocol for Frizzled 10 antibody 85829-2-RRDownload protocol
WB protocol for Frizzled 10 antibody 85829-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...