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验证数据展示

  • WB analysis using 85128-6-RR (same clone as 85128-6-PBS)

    WB analysis using 85128-6-RR (same clone as 85128-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85128-6-RR (Foxp3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85128-6-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 85128-6-RR (same clone as 85128-6-PBS)

    IHC staining of mouse spleen using 85128-6-RR (same clone as 85128-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85128-6-RR (Foxp3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85128-6-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 85128-6-RR (same clone as 85128-6-PBS)

    IHC staining of human tonsillitis using 85128-6-RR (same clone as 85128-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 85128-6-RR (Foxp3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85128-6-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 85128-6-RR (same clone as 85128-6-PBS)

    IHC staining of mouse spleen using 85128-6-RR (same clone as 85128-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85128-6-RR (Foxp3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85128-6-PBS in a different storage buffer formulation.

  • IF Staining of mouse spleen using 85128-6-RR (same clone as 85128-6-PBS)

    IF Staining of mouse spleen using 85128-6-RR (same clone as 85128-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using Foxp3 antibody (85128-6-RR, Clone: 242853G11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85128-6-PBS in a different storage buffer formulation.

  • IF Staining of mouse spleen using 85128-6-RR (same clone as 85128-6-PBS)

    IF Staining of mouse spleen using 85128-6-RR (same clone as 85128-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using Foxp3 antibody (85128-6-RR, Clone: 242853G11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85128-6-PBS in a different storage buffer formulation.

Mouse Foxp3 Recombinant monoclonal antibody, PBS Only

Foxp3 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 85128-6-PBS

产品说明书

CloneNo. 242853G11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

forkhead box P3, Forkhead box protein P3 41 kDa form, Forkhead box protein P3, C-terminally processed, JM2, scurfin

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85128-6-PBS targets Foxp3 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Eg4360

Product name: Recombinant Mouse Foxp3 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 10007378 of

Sequence: YPLLANGVCKWPGCEKVFEEPEEFLKHCQADHLLDEKGKAQCLLQREVVQSLEQQLELEKEKLGAMQAHLAGKMALAKAPS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 forkhead box P3
别名 forkhead box P3, Forkhead box protein P3 41 kDa form, Forkhead box protein P3, C-terminally processed, JM2, scurfin
观测分子量 45 kDa
基因名称 Foxp3
Gene ID (NCBI) 20371
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ99JB6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FOXP3, also named as IPEX, JM2 and Scurfin, is a transcription factor. FOXP3 plays a critical role in the control of immune response. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome.

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