验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells, A549 cells, HEK-293 cells, HeLa cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy FLOT2/Flotillin 2 Ready-To-Use IHC Kit. FLOT2/Flotillin 2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28208-1-AP targets Flotillin 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | Human |
| 文献引用反应性 | human |
| 免疫原 | Flotillin 2 fusion protein Ag28308 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | flotillin 2 |
| 别名 | ECS 1, ECS1, Epidermal surface antigen, ESA, ESA1, FLOT2, flotillin 2, M17S1 |
| 计算分子量 | 379 aa, 42 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC017292 |
| 基因名称 | Flotillin 2 |
| Gene ID (NCBI) | 2319 |
| RRID | AB_2881089 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14254 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Flotillin 2 antibody 28208-1-AP | Download protocol |
| IHC protocol for Flotillin 2 antibody 28208-1-AP | Download protocol |
| IF protocol for Flotillin 2 antibody 28208-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Commun (Lond) Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer | ||
J Exp Clin Cancer Res RNA-binding protein CCDC137 activates AKT signaling and promotes hepatocellular carcinoma through a novel non-canonical role of DGCR8 in mRNA localization | ||
Int J Biol Sci miR-34a-5p functions as a tumor suppressor in head and neck squamous cell cancer progression by targeting Flotillin-2. | ||
Int J Biol Sci TBL1X and Flot2 form a positive feedback loop to promote metastasis in nasopharyngeal carcinoma. | ||
Oncol Rep PLCD3, a flotillin2-interacting protein, is involved in proliferation, migration and invasion of nasopharyngeal carcinoma cells. | ||