×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 83598-4-RR

    WB analysis using 83598-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83598-4-RR (Flotillin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of U-251 using 83598-4-RR

    WB analysis of U-251 using 83598-4-RR

    WB result of Flotillin 1 antibody (83598-4-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Flotillin 1 transfected U-251 cells.

  • IHC staining of human stomach cancer using 83598-4-RR

    IHC staining of human stomach cancer using 83598-4-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83598-4-RR (Flotillin 1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FLOT1 antibody included
  • IHC staining of human stomach cancer using 83598-4-RR

    IHC staining of human stomach cancer using 83598-4-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83598-4-RR (Flotillin 1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FLOT1 antibody included
  • IP experiment of mouse brain using 83598-4-RR

    IP experiment of mouse brain using 83598-4-RR

    IP result of anti-Flotillin 1 (IP:83598-4-RR, 4ug; Detection:83598-4-RR 1:3000) with mouse brain tissue lysate 2400 ug.

  • IF Staining of HeLa using 83598-4-RR

    IF Staining of HeLa using 83598-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Flotillin 1 antibody (83598-4-RR, Clone: 240582G6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83598-4-RR

    Affinity and Kinetic Characterization of 83598-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83598-4-RR against Human Flotillin 1 were performed. The affinity constant is 30.5 nM.

  • Featured Product
  • KD/KO Validated

Flotillin 1 Recombinant monoclonal antibody

Flotillin 1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 83598-4-RR

产品说明书

CloneNo. 240582G6
引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

FLOT1, 240582G6, Flotillin-1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inU-87 MG cells, U-251 cells, LNCaP cells, Raji cells, mouse heart tissue, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy FLOT1 Ready-To-Use IHC Kit. FLOT1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

83598-4-RR targets Flotillin 1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原

CatNo: Ag7902

Product name: Recombinant human Flotillin 1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 80-427 aa of BC001146

Sequence: MLAAACQMFLGKTEAEIAHIALETLEGHQRAIMAHMTVEEIYKDRQKFSEQVFKVASSDLVNMGISVVSYTLKDIHDDQDYLHSLGKARTAQVQKDARIGEAEAKRDAGIREAKAKQEKVSAQYLSEIEMAKAQRDYELKKAAYDIEVNTRRAQADLAYQLQVAKTKQQIEEQRVQVQVVERAQQVAVQEQEIARREKELEARVRKPAEAERYKLERLAEAEKSQLIMQAEAEAASVRMRGEAEAFAIGARARAEAEQMAKKAEAFQLYQEAAQLDMLLEKLPQVAEEISGPLTSANKITLVSSGSGTMGAAKVTGEVLDILTRLPESVERLTGVSISQVNHKPLRTA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 flotillin 1
别名 FLOT1, 240582G6, Flotillin-1
计算分子量 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC001146
基因名称 Flotillin 1
Gene ID (NCBI) 10211
RRIDAB_3671213
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO75955
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins commonly used as lipid raft markers. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.

实验方案

Product Specific Protocols
IF protocol for Flotillin 1 antibody 83598-4-RRDownload protocol
IHC protocol for Flotillin 1 antibody 83598-4-RRDownload protocol
IP protocol for Flotillin 1 antibody 83598-4-RRDownload protocol
WB protocol for Flotillin 1 antibody 83598-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Neurochem

Young Regulatory T Cell-Derived Extracellular Vesicles Improve Mitochondrial Function and Angiogenesis and Suppress Inflammation in Senescent Brain and Heart.

Authors - Jiahui Cheng
View Article
||
New chat

Able™

正在加载，请稍候...