DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope)

DYKDDDDK tag Polyclonal Antibody for WB, IP, ELISA
Cat No. 20543-1-AP

产品说明书

引用文献(1220)

宿主/亚型

Rabbit / IgG

种属反应性

recombinant protein and More (5)

应用

WB, IP, ELISA and More (6)

Flag, DYKDDDDK, DDDDK tag, DDDDK

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 750
规格价格库存


经过测试的应用

Positive WB detected inTransfected HEK-293T cells
Positive IP detected inTransfected HEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 750 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20543-1-AP targets DYKDDDDK tag in WB, IHC, IF, IP, CoIP, ChIP, RIP, ELISA, ICC-IF applications and shows reactivity with recombinant protein samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, ChIP, RIP, ICC-IF
经测试反应性 recombinant protein
文献引用反应性human, mouse, pig, silkworm, sus scrofa (pig)
免疫原 DYKDDDDK tag fusion protein Ag2329 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Flag Tag
别名 Flag, DYKDDDDK, DDDDK tag, DDDDK
基因名称 Flag Tag
Gene ID (NCBI) 8
RRIDAB_11232216
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a rabbit polyclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (1x) and (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

实验方案

Product Specific Protocols
WB protocol for DYKDDDDK tag antibody 20543-1-APDownload protocol
IP protocol for DYKDDDDK tag antibody 20543-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Nature

7-Dehydrocholesterol dictates ferroptosis sensitivity

Authors - Yaxu Li
IP

Science

Ubiquitination of G3BP1 mediates stress granule disassembly in a context-specific manner.

Authors - Youngdae Gwon
WB,IF

Signal Transduct Target Ther

Trogocytosis of CAR molecule regulates CAR-T cell dysfunction and tumor antigen escape

Authors - You Zhai
IP

Cell

Ca2+ sensor-mediated ROS scavenging suppresses rice immunity and is exploited by a fungal effector.

Authors - Mingjun Gao
WB

Protein Cell

Senktide blocks aberrant RTN3 interactome to retard memory decline and tau pathology in social isolated Alzheimer's disease mice

Authors - He-Zhou Huang
WB,IP

Nat Metab

Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma

Authors - Zijian Yang
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