DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope)
DYKDDDDK tag Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
recombinant protein and More (1)
Applications
WB, IP, ELISA and More (5)
Conjugate
Unconjugated
1034
验证数据展示
经过测试的应用
Positive WB detected in | Transfected HEK-293T cells |
Positive IP detected in | Transfected HEK-293 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 750 conjugated versions of this antibody.
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:20000-1:100000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
20543-1-AP targets DYKDDDDK tag in WB, IHC, IF, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with recombinant protein samples.
Tested Applications | WB, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP, ChIP, RIP |
Tested Reactivity | recombinant protein |
Cited Reactivity | human |
Immunogen | DYKDDDDK tag fusion protein Ag2329 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | DYKDDDDK tag |
Synonyms | Flag, DYKDDDDK, DDDDK tag, DDDDK |
Gene Symbol | |
Gene ID (NCBI) | |
RRID | AB_11232216 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a rabbit polyclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (1x) and (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.
实验方案
Product Specific Protocols | |
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WB protocol for DYKDDDDK tag antibody 20543-1-AP | Download protocol |
IP protocol for DYKDDDDK tag antibody 20543-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
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