DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope)

DYKDDDDK tag Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein and More (9)

Applications

WB, ELISA and More (7)

Conjugate

Unconjugated

Cat No : 20543-1-AP

Print datasheet

Synonyms

DDDDK, DDDDK tag, DYKDDDDK, DYKDDDDK tag, Flag



经过测试的应用

Positive WB detected inTransfected HEK-293T cells
Planning an IF experiment? We recommend our CoraLite® Plus 750 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20543-1-AP targets DYKDDDDK tag in WB, IP, IF, FC, RIP, IHC, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB, IP, IF, FC, RIP, IHC, CoIP, ChIP
Tested Reactivity recombinant protein
Cited Reactivityhuman, mouse, rat, chicken, yeast, sheep, monkey, pig, duck
Immunogen DYKDDDDK tag fusion protein Ag2329 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DYKDDDDK tag
Synonyms DDDDK, DDDDK tag, DYKDDDDK, DYKDDDDK tag, Flag
Gene Symbol
Gene ID (NCBI)
RRIDAB_11232216
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a rabbit polyclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (1x) and (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

实验方案

Product Specific Protocols
WB protocol for DYKDDDDK tag antibody 20543-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IP

Science

Ubiquitination of G3BP1 mediates stress granule disassembly in a context-specific manner.

Authors - Youngdae Gwon
IP

Cell

Ca2+ sensor-mediated ROS scavenging suppresses rice immunity and is exploited by a fungal effector.

Authors - Mingjun Gao
humanWB

Protein Cell

Senktide blocks aberrant RTN3 interactome to retard memory decline and tau pathology in social isolated Alzheimer's disease mice

Authors - He-Zhou Huang
WB,IP

Nat Metab

Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma

Authors - Zijian Yang
WB

Cell Res

Cholesterylation of Smoothened is a calcium-accelerated autoreaction involving an intramolecular ester intermediate.

Authors - Ao Hu

Cell Res

Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.

Authors - Fei Peng