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Fhl1 Polyclonal antibody

Fhl1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 51015-1-AP

Print datasheet

Synonyms

Fhl1, four and a half LIM domains 1, KyoT

验证数据展示

  • WB analysis of human heart using 51015-1-AP

    WB analysis of human heart using 51015-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 51015-1-AP (Fhl1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 51015-1-AP

    IHC staining of human ovary tumor using 51015-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 51015-1-AP (Fhl1 antibody) at dilution of 1:50 (under 10x lens).

经过测试的应用

Positive WB detected inhuman heart tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51015-1-AP targets Fhl1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB,ELISA
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name four and a half LIM domains 1
Synonyms Fhl1, four and a half LIM domains 1, KyoT
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC031120
Gene Symbol Fhl1
Gene ID (NCBI) 14199
RRIDAB_615005
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP97447
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Fhl1 antibody 51015-1-APDownload protocol
IHC protocol for Fhl1 antibody 51015-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols