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验证数据展示

  • WB analysis of mouse testis using 84731-7-RR

    WB analysis of mouse testis using 84731-7-RR

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 84731-7-RR (Ferritin light chain 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84731-7-RR

    WB analysis using 84731-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84731-7-RR (Ferritin light chain 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat liver using 84731-7-RR

    WB analysis of rat liver using 84731-7-RR

    rat liver tissue were subjected to SDS PAGE followed by western blot with 84731-7-RR (Ferritin light chain 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 84731-7-RR

    IHC staining of mouse liver using 84731-7-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84731-7-RR (Ferritin light chain 1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse liver using 84731-7-RR

    IF Staining of mouse liver using 84731-7-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Ferritin light chain 1 antibody (84731-7-RR, Clone: 242299F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Ferritin light chain 1 Recombinant monoclonal antibody

Ferritin light chain 1 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 84731-7-RR

产品说明书

CloneNo. 242299F7

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

242299F7, Ferritin L subunit 1, Ftl, Ftl1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue, mouse liver tissue, rat liver tissue, mouse spleen tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84731-7-RR targets Ferritin light chain 1 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg2434

Product name: Recombinant Rat Ferritin light chain 1 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 1-183 aa of NM_022500.4

Sequence: MTSQIRQNYSTEVEAAVNRLVNLHLRASYTYLSLGFFFDRDDVALEGVGHFFRELAEEKREGAERLLKLQNERGGRALFQDVQKPSQDEWGKTLEAMEAALALEKNLNQALLDLHALGSARTDPHLCDFLESHFLDKEVKLIKKMGNHLTNLRRVAGPQPAQTGVAQASLGEYLFERLTLKHD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Ferritin light chain 1
别名 242299F7, Ferritin L subunit 1, Ftl, Ftl1
计算分子量21 kDa
观测分子量18-20 kDa
GenBank蛋白编号NM_022500.4
基因名称 Ftl
Gene ID (NCBI) 29292
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP02793
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FTL belongs to the ferritin family. It stores iron in a soluble, non-toxic, readily available form. FTL is important for iron homeostasis. It plays a role in delivery of iron to cells. FTL mediates iron uptake in capsule cells of the developing kidney. Mutation of FTL will cause hereditary hyperferritinemia-cataract syndrome (HHCS) or neurodegeneration with brain iron accumulation type 3 (NBIA3). Ferritin light polypeptide (FTL) and ferritin heavy polypeptide (FTH1) were the main constituents the striatum and cerebellar cortex revealed.

实验方案

Product Specific Protocols
IHC protocol for Ferritin light chain 1 antibody 84731-7-RRDownload protocol
WB protocol for Ferritin light chain 1 antibody 84731-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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