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Fas/CD95 Polyclonal antibody

Fas/CD95 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 30601-1-AP

Print datasheet

Synonyms

AI196731, APO 1, APT1, CD95, Fas, lpr, TNFR6, Tnfrsf6

验证数据展示

  • WB analysis using 30601-1-AP

    WB analysis using 30601-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30601-1-AP (Fas/CD95 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 30601-1-AP

    WB analysis using 30601-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30601-1-AP (Fas/CD95 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of Raji using 30601-1-AP

    IF Staining of Raji using 30601-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using Fas/CD95 antibody (30601-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, J774A.1 cells, mouse brain tissue, rat brain tissue
Positive IF detected inRaji cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30601-1-AP targets Fas/CD95 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen Fas/CD95 fusion protein Ag30426 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Fas (TNF receptor superfamily member 6)
Synonyms AI196731, APO 1, APT1, CD95, Fas, lpr, TNFR6, Tnfrsf6
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 30-40 kDa
GenBank Accession NumberNM_007987
Gene Symbol Fas
Gene ID (NCBI) 14102
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP25446
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAS, also named as CD95, APO-1, APT1, FAS1 and TNFRSF6, is a receptor for TNFSF6/FASLG. It is a cell surface receptor belonging to the TNF receptor superfamily, can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.

实验方案

Product Specific Protocols
WB protocol for Fas/CD95 antibody 30601-1-APDownload protocol
IF protocol for Fas/CD95 antibody 30601-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols