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验证数据展示

  • WB analysis of human plasma using 66753-1-Ig

    WB analysis of human plasma using 66753-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66753-1-Ig (Factor X antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 66753-1-Ig

    IHC staining of human liver using 66753-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66753-1-Ig (Factor X antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 66753-1-Ig

    IHC staining of human liver using 66753-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66753-1-Ig (Factor X antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human liver using 66753-1-Ig

    IF Staining of human liver using 66753-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Factor X antibody (66753-1-Ig, Clone: 3D3F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Factor X Monoclonal antibody

Factor X Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66753-1-Ig

产品说明书

CloneNo. 3D3F11

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF-P, ELISA

别名

F10, Factor X heavy chain, EC:3.4.21.6, coagulation factor X, Activated factor Xa heavy chain

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66753-1-Ig targets Factor X in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原 Factor X fusion protein Ag11664 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 coagulation factor X
别名 F10, Factor X heavy chain, EC:3.4.21.6, coagulation factor X, Activated factor Xa heavy chain
计算分子量 488 aa, 55 kDa
观测分子量 50 kDa
GenBank蛋白编号BC046125
基因名称 F10
Gene ID (NCBI) 2159
RRIDAB_2882099
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP00742
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Coagulation factor X (F10) is a vitamin K-dependent glycoprotein that participates in the middle phase of the blood coagulation cascade (PMID: 1931959; 1313796). It is synthesized as a single-chain precursor in the liver where it undergoes a number of covalent processing steps before secretion into the blood as a two-chain molecule linked by a disulfide bond (PMID: 1313796). The light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. Factor X is activated into factor Xa, by both the extrinsic and intrinsic pathway. The activated factor then converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.

实验方案

Product Specific Protocols
WB protocol for Factor X antibody 66753-1-IgDownload protocol
IHC protocol for Factor X antibody 66753-1-IgDownload protocol
IF protocol for Factor X antibody 66753-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols