• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of human plasma using 84533-4-RR (same clone as 84533-4-PBS)

    WB analysis of human plasma using 84533-4-RR (same clone as 84533-4-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 84533-4-RR (Coagulation factor IX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84533-4-PBS in a different storage buffer formulation.

  • IHC staining of Human Hepatocellular cancer using 84533-4-RR (same clone as 84533-4-PBS)

    IHC staining of Human Hepatocellular cancer using 84533-4-RR (same clone as 84533-4-PBS)

    Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 84533-4-RR (Factor IX/PTC antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84533-4-PBS in a different storage buffer formulation.

Factor IX/PTC Recombinant antibody, PBS Only

Factor IX/PTC Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84533-4-PBS

产品说明书

CloneNo. 241742G4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

F9, Factor IX, Christmas factor, coagulation factor IX, Coagulation factor IXa heavy chain

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

84533-4-PBS targets Factor IX/PTC in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 Factor IX/PTC fusion protein Eg1045 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 coagulation factor IX
别名 F9, Factor IX, Christmas factor, coagulation factor IX, Coagulation factor IXa heavy chain
计算分子量52kDa
观测分子量 72 kDa
GenBank蛋白编号NM_000133.4
基因名称 F9
Gene ID (NCBI) 2158
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP00740-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The F9 gene encodes coagulation factor IX, which circulates as an inactive zymogen until proteolytic release of its activation peptide allows it to assume the conformation of an active serine protease. Its role in the blood coagulation cascade is to activate factor X (F10) through interactions with calcium, membrane phospholipids, and factor VIII (F8). Factor IX and factor X both consist of 2 polypeptide chains referred to as the L (light) and H (heavy) chains. The H chain bears a structural resemblance to the polypeptide chain of the pancreatic serine protease trypsin (PRSS1). The L chain is covalently linked to the H chain by a single disulfide bond. Native Factor IX MW is around 72kDa(PMID:827443; 12496253), and it can be cleaved into different fragments.