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验证数据展示

  • WB analysis using 16368-1-AP

    WB analysis using 16368-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16368-1-AP (FZR1/Cdh1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16368-1-AP

    IHC staining of human liver using 16368-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16368-1-AP (FZR1 antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with FZR1 antibody included
  • IHC staining of human liver using 16368-1-AP

    IHC staining of human liver using 16368-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16368-1-AP (FZR1 antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with FZR1 antibody included
  • IF Staining of HepG2 using 16368-1-AP

    IF Staining of HepG2 using 16368-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 16368-1-AP (FZR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of mouse heart using 16368-1-AP

    IP experiment of mouse heart using 16368-1-AP

    IP result of anti-FZR1/Cdh1 (IP:16368-1-AP, 4ug; Detection:16368-1-AP 1:500) with mouse heart tissue lysate 3200ug.

  • FC experiment of HeLa using 16368-1-AP

    FC experiment of HeLa using 16368-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human FZR1/Cdh1 (16368-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IHC staining of mouse heart using 16368-1-AP

    IHC staining of mouse heart using 16368-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16368-1-AP (FZR1/Cdh1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FZR1 antibody included
  • IHC staining of mouse kidney using 16368-1-AP

    IHC staining of mouse kidney using 16368-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16368-1-AP (FZR1/Cdh1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FZR1 antibody included
  • WB analysis using 16368-1-AP

    WB analysis using 16368-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16368-1-AP (FZR1/Cdh1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 16368-1-AP

    IHC staining of mouse liver using 16368-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16368-1-AP (FZR1/Cdh1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FZR1 antibody included
  • IHC staining of human ovary cancer using 16368-1-AP

    IHC staining of human ovary cancer using 16368-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16368-1-AP (FZR1/Cdh1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FZR1 antibody included
  • IHC staining of mouse kidney using 16368-1-AP

    IHC staining of mouse kidney using 16368-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16368-1-AP (FZR1/Cdh1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FZR1 antibody included
  • IHC staining of rat heart using 16368-1-AP

    IHC staining of rat heart using 16368-1-AP

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 16368-1-AP (FZR1/Cdh1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FZR1 antibody included
  • IHC staining of rat kidney using 16368-1-AP

    IHC staining of rat kidney using 16368-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 16368-1-AP (FZR1/Cdh1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FZR1 antibody included
  • IHC staining of rat liver using 16368-1-AP

    IHC staining of rat liver using 16368-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 16368-1-AP (FZR1/Cdh1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FZR1 antibody included
  • Featured Product
  • KD/KO Validated

FZR1/Cdh1 Polyclonal antibody

FZR1/Cdh1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 16368-1-AP

产品说明书

引用文献(15)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

别名

FZR1, CDC20 like protein 1, CDC20C, CDC20-like protein 1, CDH1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHMy2.CIR cells, C6 cells, HEK-293 cells, HepG2 cells, mouse liver tissue, HeLa cells, NIH/3T3 cells, mouse heart tissue, rat heart tissue, Jurkat cells
Positive IP detected inmouse heart tissue
Positive IHC detected inmouse kidney tissue, rat liver tissue, mouse kidney tissue, mouse heart tissue, rat heart tissue, rat kidney tissue, human liver tissue, human breast cancer tissue, mouse liver tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy® FZR1 Ready-To-Use IHC Kit. FZR1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16368-1-AP targets FZR1/Cdh1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag9201

Product name: Recombinant human FZR1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 217-342 aa of BC013413

Sequence: SQVTRLCDLSVEGDSVTSVGWSERGNLVAVGTHKGFVQIWDAAAGKKLSMLEGHTARVGALAWNAEQLSSGSRDRMILQRDIRTPPLQSERRLQGHRQEVCGLKWSTDHQLLASGGNDNKLLVWNH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fizzy/cell division cycle 20 related 1 (Drosophila)
别名 FZR1, CDC20 like protein 1, CDC20C, CDC20-like protein 1, CDH1
计算分子量 493 aa, 55 kDa
观测分子量 55 kDa
GenBank蛋白编号BC013413
基因名称 FZR1
Gene ID (NCBI) 51343
RRIDAB_2107380
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UM11
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FZR1 gene, also named as FYR, FZR and KIAA1242, has been previously shown to be mutated in lobular breast carcinomas. Direct evidence of FZR1 mutations triggering tumorigenesis has been investigated. FZR1 is a key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C) which is playing vital role s in cell cycle progression. There are 7 WD-repeat domains in FZR1. Upon phosphorylation and dephosphorylation, it interacts with other components to modulate cell mitosis. This gene is different to E-cadherin (CDH1)which is an epithelial cell adhesion molecule. It has 55 kDa, 54 kDa and 45 kDa isoforms.

实验方案

Product Specific Protocols
FC protocol for FZR1/Cdh1 antibody 16368-1-APDownload protocol
IF protocol for FZR1/Cdh1 antibody 16368-1-APDownload protocol
IHC protocol for FZR1/Cdh1 antibody 16368-1-APDownload protocol
IP protocol for FZR1/Cdh1 antibody 16368-1-APDownload protocol
WB protocol for FZR1/Cdh1 antibody 16368-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Signal Transduct Target Ther

Phosphorylation determines the glucose metabolism reprogramming and tumor-promoting activity of sine oculis homeobox 1

Authors - Yanni Lin
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humanWB

Nat Cell Biol

Phase separation of ERCC6L2-CtIP regulates the extent of DNA end resection

Authors - Yixin Yin
  • KD Validated
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humanWB

Nat Commun

DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation

Authors - Xiaohua Xu
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humanWB

Nat Chem Biol

Molecular recording of cellular protein kinase activity with chemical labeling

Authors - De-En Sun
  • KD Validated
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Adv Sci (Weinh)

CDH1 Orchestrates Anabolic Events to Promote Cell Cycle Initiation

Authors - Yun-Zi Mao
View Article
mouse,humanWB

Adv Sci (Weinh)

CDC20-Mediated Selective Autophagy Degradation of PBRM1 Affects Immunotherapy for Renal Cell Carcinoma

Authors - Yizeng Fan
View Article
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