验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, HL-60 cells, NCI-H1299 cells, U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28326-1-AP targets FZD1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag28500 Product name: Recombinant human FZD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 210-322 aa of BC051271 Sequence: PDTLKCEKFPVHGAGELCVGQNTSDKGTPTPSLLPEFWTSNPQHGGGGHRGGFPGGAGASERGKFSCPRALKVPSYLNYHFLGEKDCGAPCEPTKVYGLMYFGPEELRFSRTW 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | frizzled homolog 1 (Drosophila) |
| 别名 | Frizzled 1, Frizzled-1, Fz 1, Fz-1, FzE1 |
| 计算分子量 | 647 aa, 71 kDa |
| 观测分子量 | 71 kDa |
| GenBank蛋白编号 | BC051271 |
| 基因名称 | FZD1 |
| Gene ID (NCBI) | 8321 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UP38 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Frizzled-1 (FZD1) is a member of the Frizzled family of transmembrane receptors, which belongs to the class F of G protein-coupled receptors (GPCRs). In mammals, this family consists of ten isoforms (FZD1-10) (PMID: 38594145). It functions as a receptor for Wnt proteins, a family of secreted lipoglycoproteins. FZD1 is activated by specific Wnt ligands such as WNT3A, WNT3, and WNT1, but not by others like WNT4, WNT5A, or WNT7A. FZD1 regulates specific stages of adult hippocampal neurogenesis, being required for neuronal differentiation and positioning of newborn neurons into the granule cell layer, but not for morphological development of adult-born granule neurons (PMID: 26980182).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FZD1 antibody 28326-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
