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验证数据展示

  • WB analysis using 17237-1-AP

    WB analysis using 17237-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17237-1-AP (FYN antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat testis using 17237-1-AP

    IHC staining of rat testis using 17237-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17237-1-AP (FYN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FYN antibody included
  • IHC staining of rat testis using 17237-1-AP

    IHC staining of rat testis using 17237-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17237-1-AP (FYN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FYN antibody included

FYN Polyclonal antibody

FYN Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17237-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Proto-oncogene c-Fyn, Proto oncogene Syn, Proto oncogene c Fyn, p59-Fyn, p59 Fyn

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, HeLa cells, K-562 cells
Positive IHC detected inrat testis tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy FYN Ready-To-Use IHC Kit. FYN primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17237-1-AP targets FYN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 FYN fusion protein Ag11080 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 FYN oncogene related to SRC, FGR, YES
别名 Proto-oncogene c-Fyn, Proto oncogene Syn, Proto oncogene c Fyn, p59-Fyn, p59 Fyn
计算分子量537aa,61 kDa; 482aa,55 kDa
观测分子量60 kDa
GenBank蛋白编号BC032496
基因名称 FYN
Gene ID (NCBI) 2534
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP06241
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FYN, also named p59-Fyn and SLK, belongs to the protein kinase superfamily, Tyr protein kinase family, and SRC subfamily. FYN is implicated in the control of cell growth. It plays a role in the regulation of intracellular calcium levels, with isoform 2 showing a greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. FYN is required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. FYN blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC.

实验方案

Product Specific Protocols
WB protocol for FYN antibody 17237-1-APDownload protocol
IHC protocol for FYN antibody 17237-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols