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验证数据展示

  • WB analysis of mouse brain using 11465-1-AP

    WB analysis of mouse brain using 11465-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11465-1-AP (FXYD7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

FXYD7 Polyclonal antibody

FXYD7 Polyclonal Antibody for WB, ELISA
Cat No. 11465-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, ELISA

别名

FXYD7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11465-1-AP targets FXYD7 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag2030

Product name: Recombinant human FXYD7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-80 aa of BC018619

Sequence: MATPTQTPTKAPEEPDPFYYDYNTVQTVGMTLATILFLLGILIVISKKVKCRKADSRSESPTCKSCKSELPSSAPGGGGV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 FXYD domain containing ion transport regulator 7
别名 FXYD7
计算分子量 9 kDa
观测分子量 18-20 kDa
GenBank蛋白编号BC018619
基因名称 FXYD7
Gene ID (NCBI) 53822
RRIDAB_2108627
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP58549
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FXYD7 (FXYD domain containing ion transport regulator 7) is a single-pass membrane protein that belongs to the FXYD family. The FXYD family, which contains seven members, are tissue specific regulators of the Na,K-ATPase. Expressed exclusively in the brain, FXYD7 is an isoform-specific Na,K-ATPase regulator which could play an important role in neuronal excitability. It has been reported that FXYD7 bears post-translationally added modifications on threonine residues and has an apparent molecular weight of 18 kDa (PMID: 12093728).

实验方案

Product Specific Protocols
WB protocol for FXYD7 antibody 11465-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols