验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, A375 cells, SGC-7901 cells |
| Positive IP detected in | COLO 320 cells |
| Positive IHC detected in | human breast cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15853-1-AP targets FXYD3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag8672 Product name: Recombinant human FXYD3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-87 aa of BC005238 Sequence: MQKVTLGLLVFLAGFPVLDANDLEDKNSPFYYDWHSLQVGGLICAGVLCAMGIIIVMSAKCKCKFGQKSGHHPGETPPLITPGSAQS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | FXYD domain containing ion transport regulator 3 |
| 别名 | Chloride conductance inducer protein Mat-8, FXYD domain-containing ion transport regulator 3, Mammary tumor 8 kDa protein, MAT 8, MAT8 |
| 计算分子量 | 87 aa, 9 kDa |
| 观测分子量 | 8 kDa |
| GenBank蛋白编号 | BC005238 |
| 基因名称 | FXYD3 |
| Gene ID (NCBI) | 5349 |
| RRID | AB_2108602 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14802 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FXYD3 (also known as Mat-8) is one of the seven members of Fxyd3 protein family, belonging to a small molecular auxiliary subunit with single transmembrane, and its extracellular segment contains conservative Fxyd3 sequence motifs. By combining the α/β catalytic core of Na/K-ATPase, this family can finely adjust the ionic affinity and kinetics of the pump in different tissues, cell types and physiological states, without affecting the overall expression of the enzyme. It is mainly expressed in stomach, colon, pancreas, prostate, skin and other epithelium, showing obvious tissue specificity, and is significantly up-regulated in breast cancer, pancreatic cancer, colorectal cancer, prostate cancer, renal cell cancer, cholangiocarcinoma and psoriasis lesions, and is related to disease progression and chemotherapy resistance.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for FXYD3 antibody 15853-1-AP | Download protocol |
| IP protocol for FXYD3 antibody 15853-1-AP | Download protocol |
| WB protocol for FXYD3 antibody 15853-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
