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- KD/KO Validated
FXR1 Polyclonal antibody
FXR1 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
IF/ICC, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse heart tissue, NIH/3T3 cells |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | Ethacrynic acid treated HepG2 cells, HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13194-1-AP targets FXR1 in WB, IP, IF, RIP, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IP, IF, RIP, IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Immunogen | FXR1 fusion protein Ag3873 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | fragile X mental retardation, autosomal homolog 1 |
| Synonyms | FXR1, hFXR1p |
| Calculated Molecular Weight | 621 aa, 70 kDa |
| Observed Molecular Weight | 70-80 kDa |
| GenBank Accession Number | BC028983 |
| Gene Symbol | FXR1 |
| Gene ID (NCBI) | 8087 |
| RRID | AB_2110702 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51114 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Tumour necrosis factor-alpha (TNF-alpha) is a key mediator of inflammation in host defence against infection and in autoimmune disease. Its production is controlled post-transcriptionally by multiple RNA-binding proteins that interact with the TNF-alpha AU-rich element and regulate its expression; Fragile X mental retardation-related protein 1 (FXR1) is one of these. FXR1 (fragile-X-mental retardation-related protein 1) are RNA-binding proteins that have been demonstrated to impact miRNA-mediated, post-transcriptional gene regulation, and required for embryonic and postnatal development of muscle tissue. It can regulate intracellular transport and local translation of certain mRNAs. The FXR1 exists some isoforms with the MW 69 kDa and 60 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FXR1 antibody 13194-1-AP | Download protocol |
| IHC protocol for FXR1 antibody 13194-1-AP | Download protocol |
| IF protocol for FXR1 antibody 13194-1-AP | Download protocol |
| IP protocol for FXR1 antibody 13194-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization. | ||
Neuron Fmr1 KO and fenobam treatment differentially impact distinct synapse populations of mouse neocortex. | ||
Nat Commun Microtubule-based transport is essential to distribute RNA and nascent protein in skeletal muscle | ||
Elife Sub-synaptic, multiplexed analysis of proteins reveals Fragile X related protein 2 is mislocalized in Fmr1 KO synapses. | ||
Elife The Tudor SND1 protein is an m6A RNA reader essential for replication of Kaposi's sarcoma-associated herpesvirus. | ||
J Neurosci Dysregulated RNA-Induced Silencing Complex (RISC) Assembly within CNS Corresponds with Abnormal miRNA Expression during Autoimmune Demyelination. |
