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验证数据展示

  • WB analysis of MDA-MB-231 using 66118-1-Ig (same clone as 66118-1-PBS)

    WB analysis of MDA-MB-231 using 66118-1-Ig (same clone as 66118-1-PBS)

    MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 66118-1-Ig (FUT8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 66118-1-Ig (same clone as 66118-1-PBS)

    WB analysis of MCF-7 using 66118-1-Ig (same clone as 66118-1-PBS)

    WB result of FUT8 antibody (66118-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FUT8 transfected MCF-7 cells. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66118-1-Ig (same clone as 66118-1-PBS)

    IHC staining of human colon cancer using 66118-1-Ig (same clone as 66118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66118-1-Ig (FUT8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66118-1-Ig (same clone as 66118-1-PBS)

    IHC staining of human colon cancer using 66118-1-Ig (same clone as 66118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66118-1-Ig (FUT8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 66118-1-Ig (same clone as 66118-1-PBS)

    IHC staining of mouse cerebellum using 66118-1-Ig (same clone as 66118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66118-1-Ig (FUT8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 66118-1-Ig (same clone as 66118-1-PBS)

    IHC staining of mouse kidney using 66118-1-Ig (same clone as 66118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66118-1-Ig (FUT8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

FUT8 Monoclonal antibody, PBS Only

FUT8 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66118-1-PBS

产品说明书

CloneNo. 2F7H2

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

2F7H2, Alpha-(1,6)-fucosyltransferase, alpha1 6FucT, Alpha1-6FucT, EC:2.4.1.68

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66118-1-PBS targets FUT8 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag18769

Product name: Recombinant human FUT8 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 276-575 aa of BC025385

Sequence: HWSGEVKDKNVQVVEFPIVDSLHPRPPYLPLAVPEDLADRLVRVHGDPAVWWVSQFVKYLIRPQPWLEKEIEEATKKLGFKHPVIGVHVRRTDKVGTEAAFHPIEEYMVHVEEHFQLLARRMQVDKKRVYLATDDPSLLKEAKTKYPNYEFISDNSISWSAGLHNRYTENSLRGVILDIHFLSQADFLVCTFSSQVCRVAYEIMQTLHPDASANFHSLDDIYYFGGQNAHNQIAIYAHQPRTADEIPMEPGDIIGVAGNHWDGYSKGVNRKLGRTGLYPSYKVREKIETVKYPTYPEAEK

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 fucosyltransferase 8 (alpha (1,6) fucosyltransferase)
别名 2F7H2, Alpha-(1,6)-fucosyltransferase, alpha1 6FucT, Alpha1-6FucT, EC:2.4.1.68
计算分子量 66 kDa
观测分子量 66 kDa
GenBank蛋白编号BC025385
基因名称 FUT8
Gene ID (NCBI) 2530
RRIDAB_2881517
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9BYC5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fucosyltransferase 8 (FUT8) is GDP-fucose-glycoprotein fucosyltransferase and is localized in Golgi apparatus. FUT8 is involved in protein glycosylation by catalyzing the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans. FUT8 may be involved in human aging by regulating protein N-glycosylation and in the molecular pathogenesis of human pulmonary emphysema (PE).

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