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验证数据展示

  • WB analysis using 29430-1-AP

    WB analysis using 29430-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29430-1-AP (FUT8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 29430-1-AP

    IHC staining of human lung cancer using 29430-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29430-1-AP (FUT8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 29430-1-AP

    IHC staining of human breast cancer using 29430-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29430-1-AP (FUT8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 29430-1-AP

    IHC staining of mouse cerebellum using 29430-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29430-1-AP (FUT8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 29430-1-AP

    IHC staining of mouse cerebellum using 29430-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29430-1-AP (FUT8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FUT8 Polyclonal antibody

FUT8 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29430-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Alpha-(1,6)-fucosyltransferase, alpha1 6FucT, Alpha1-6FucT, EC:2.4.1.68, FUT 8

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMDA-MB-231 cells, NCI-H1299 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29430-1-AP targets FUT8 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag30784

Product name: Recombinant human FUT8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 340-450 aa of BC025385

Sequence: PWLEKEIEEATKKLGFKHPVIGVHVRRTDKVGTEAAFHPIEEYMVHVEEHFQLLARRMQVDKKRVYLATDDPSLLKEAKTKYPNYEFISDNSISWSAGLHNRYTENSLRGV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fucosyltransferase 8 (alpha (1,6) fucosyltransferase)
别名 Alpha-(1,6)-fucosyltransferase, alpha1 6FucT, Alpha1-6FucT, EC:2.4.1.68, FUT 8
计算分子量 66 kDa
观测分子量60-66 kDa
GenBank蛋白编号BC025385
基因名称 FUT8
Gene ID (NCBI) 2530
RRIDAB_2918305
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BYC5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for FUT8 antibody 29430-1-APDownload protocol
WB protocol for FUT8 antibody 29430-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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