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验证数据展示

  • WB analysis of human placenta using 67344-1-Ig

    WB analysis of human placenta using 67344-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67344-1-Ig (FUT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of human prostate hyperplasia using 67344-1-Ig

    IF Staining of human prostate hyperplasia using 67344-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate hyperplasia tissue using FUT3 antibody (67344-1-Ig, Clone: 2A6G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human prostate hyperplasia using 67344-1-Ig

    IF Staining of human prostate hyperplasia using 67344-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate hyperplasia tissue using FUT3 antibody (67344-1-Ig, Clone: 2A6G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human colon cancer using 67344-1-Ig

    IHC staining of human colon cancer using 67344-1-Ig

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 67344-1-Ig (FUT3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FUT3 Monoclonal antibody

FUT3 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67344-1-Ig

产品说明书

CloneNo. 2A6G7
引用文献(2)

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF-P, ELISA

别名

3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase FUT3, 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase, Alpha-3-fucosyltransferase FUT3, Blood group Lewis alpha-4-fucosyltransferase, CD174

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman placenta tissue, NCCIT cells, JAR cells, HepG2 cells
Positive IHC detected inhuman colon cancer tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman prostate hyperplasia tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67344-1-Ig targets FUT3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag12581

Product name: Recombinant human FUT3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 84-361 aa of BC108675

Sequence: MVPGTADCHITADRKVYPQADTVIVHHWDIMSNPKSRLPPSPRPQGQRWIWFNLEPPPNCQHLEALDRYFNLTMSYRSDSDIFTPYGWLEPWSGQPAHPPLNLSAKTELVAWAVSNWKPDSARVRYYQSLQAHLKVDVYGRSHKPLPKGTMMETLSRYKFYLAFENSLHPDYITEKLWRNALEAWAVPVVLGPSRSNYERFLPPDAFIHVDDFQSPKDLARYLQELDKDHARYLSYFRWRETLRPRSFSWALDFCKACWKLQQESRYQTVRSIAAWFT

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group)
别名 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase FUT3, 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase, Alpha-3-fucosyltransferase FUT3, Blood group Lewis alpha-4-fucosyltransferase, CD174
计算分子量 361 aa, 42 kDa
观测分子量 42-45 kDa
GenBank蛋白编号BC108675
基因名称 FUT3
Gene ID (NCBI) 2525
RRIDAB_2882602
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP21217
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for FUT3 antibody 67344-1-IgDownload protocol
IHC protocol for FUT3 antibody 67344-1-IgDownload protocol
WB protocol for FUT3 antibody 67344-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

NPJ Precis Oncol

FUT3 mediated GRP78 fucosylation promotes colorectal cancer survival and proliferation under glucose restriction via PERK/ATF4/STC2 axis.

Authors - Kaiwen Qin
View Article
humanWB,IHC

Eur J Med Res

FUT3-B3GNT3 interaction promotes pancreatic cancer progression and chemoresistance via NF-κB signaling mediated autophagy.

Authors - Lei Jin
  • KD Validated
View Article
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