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验证数据展示

  • WB analysis of human placenta using 67344-1-Ig

    WB analysis of human placenta using 67344-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67344-1-Ig (FUT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of human prostate hyperplasia using 67344-1-Ig

    IF Staining of human prostate hyperplasia using 67344-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate hyperplasia tissue using FUT3 antibody (67344-1-Ig, Clone: 2A6G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human prostate hyperplasia using 67344-1-Ig

    IF Staining of human prostate hyperplasia using 67344-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate hyperplasia tissue using FUT3 antibody (67344-1-Ig, Clone: 2A6G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human colon cancer using 67344-1-Ig

    IHC staining of human colon cancer using 67344-1-Ig

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 67344-1-Ig (FUT3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FUT3 Monoclonal antibody

FUT3 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67344-1-Ig

产品说明书

CloneNo. 2A6G7

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF-P, ELISA

别名

3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase FUT3, 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase, Alpha-3-fucosyltransferase FUT3, Blood group Lewis alpha-4-fucosyltransferase, CD174

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman placenta tissue, NCCIT cells, JAR cells, HepG2 cells
Positive IHC detected inhuman colon cancer tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman prostate hyperplasia tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67344-1-Ig targets FUT3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag12581

Product name: Recombinant human FUT3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 84-361 aa of BC108675

Sequence: MVPGTADCHITADRKVYPQADTVIVHHWDIMSNPKSRLPPSPRPQGQRWIWFNLEPPPNCQHLEALDRYFNLTMSYRSDSDIFTPYGWLEPWSGQPAHPPLNLSAKTELVAWAVSNWKPDSARVRYYQSLQAHLKVDVYGRSHKPLPKGTMMETLSRYKFYLAFENSLHPDYITEKLWRNALEAWAVPVVLGPSRSNYERFLPPDAFIHVDDFQSPKDLARYLQELDKDHARYLSYFRWRETLRPRSFSWALDFCKACWKLQQESRYQTVRSIAAWFT

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group)
别名 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase FUT3, 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase, Alpha-3-fucosyltransferase FUT3, Blood group Lewis alpha-4-fucosyltransferase, CD174
计算分子量 361 aa, 42 kDa
观测分子量 42-45 kDa
GenBank蛋白编号BC108675
基因名称 FUT3
Gene ID (NCBI) 2525
RRIDAB_2882602
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP21217
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for FUT3 antibody 67344-1-IgDownload protocol
IHC protocol for FUT3 antibody 67344-1-IgDownload protocol
WB protocol for FUT3 antibody 67344-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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