验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, L02 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human testis tissue, human brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy FUT11 Ready-To-Use IHC Kit. FUT11 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17175-1-AP targets FUT11 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag10815 Product name: Recombinant human FUT11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 143-492 aa of BC036037 Sequence: ALLHEESPLNNFLLSHGPGIRLFNLTSTFSRHSDYPLSLQWLPGTAYLRRPVPPPMERAEWRRRGYAPLLYLQSHCDVPADRDRYVRELMRHIPVDSYGKCLQNRELPTARLQDTATATTEDPELLAFLSRYKFHLALENAICNDYMTEKLWRPMHLGAVPVYRGSPSVRDWMPNNHSVILIDDFESPQKLAEFIDFLDKNDEEYMKYLAYKQPGGITNQFLLDSLKHREWGVNDPLLPNYLNGFECFVCDYELARLDAEKAHAASPGDSPVFEPHIAQPSHMDCPVPTPGFGNVEEIPENDSWKEMWLQDYWQGLDQGEALTAMIHNNETEQTKFWDYLHEIFMKRQHL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | fucosyltransferase 11 (alpha (1,3) fucosyltransferase) |
| 别名 | Alpha-(1,3)-fucosyltransferase 11, EC:2.4.1.-, Fuc TXI, FucT XI, FucTXI |
| 计算分子量 | 492 aa, 56 kDa |
| 观测分子量 | 56 kDa |
| GenBank蛋白编号 | BC036037 |
| 基因名称 | FUT11 |
| Gene ID (NCBI) | 170384 |
| RRID | AB_2231850 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q495W5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for FUT11 antibody 17175-1-AP | Download protocol |
| WB protocol for FUT11 antibody 17175-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int Immunopharmacol Dry and wet experiments reveal the significant role of FUT11 in clear cell renal cell carcinoma | ||
Cell Biol Int FUT11 is a target gene of HIF1α that promotes the progression of hepatocellular carcinoma. | ||
Heliyon Knockdown of FUT11 inhibits the progression of gastric cancer via the PI3K/AKT pathway | ||
Discov Oncol FUT11 expression in gastric cancer: its prognostic significance and role in immune regulation | ||
Nat Chem Biol FUT10 and FUT11 are protein O-fucosyltransferases that modify protein EMI domains | ||
BMC Cancer Fucosyltransferase 11 restrains ferroptosis via upregulation GPX4 expression in gastric cancer |
