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验证数据展示

  • IP experiment of mouse heart using 15157-1-AP (same clone as 15157-1-PBS)

    IP experiment of mouse heart using 15157-1-AP (same clone as 15157-1-PBS)

    IP result of anti-FUCA2 (IP:15157-1-AP, 4ug; Detection:15157-1-AP 1:300) with mouse heart tissue lysate 4000 ug. This data was developed using the same antibody clone with 15157-1-PBS in a different storage buffer formulation.

  • WB analysis of human heart using 15157-1-AP (same clone as 15157-1-PBS)

    WB analysis of human heart using 15157-1-AP (same clone as 15157-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 15157-1-AP (FUCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15157-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 15157-1-AP (same clone as 15157-1-PBS)

    IHC staining of human liver using 15157-1-AP (same clone as 15157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 15157-1-AP (FUCA2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15157-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 15157-1-AP (same clone as 15157-1-PBS)

    IHC staining of human liver using 15157-1-AP (same clone as 15157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 15157-1-AP (FUCA2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15157-1-PBS in a different storage buffer formulation.

FUCA2 Polyclonal antibody, PBS Only

FUCA2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 15157-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15157-1-PBS targets FUCA2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7250

Product name: Recombinant human FUCA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 124-467 aa of BC003060

Sequence: SGAKYIVLTSKHHEGFTLWGSEYSWNWNAIDEGPKRDIVKELEVAIRNRTDLRFGLYYSLFEWFHPLFLEDESSSFHKRQFPVSKTLPELYELVNNYQPEVLWSDGDGGAPDQYWNSTGFLAWLYNESPVRGTVVTNDRWGAGSICKHGGFYTCSDRYNPGHLLPHKWENCMTIDKLSWGYRREAGISDYLTIEELVKQLVETVSCGGNLLMNIGPTLDGTISVVFEERLRQVGSWLKVNGEAIYETYTWRSQNDTVTPDVWYTSKPKEKLVYAIFLKWPTSGQLFLGHPKAILGATEVKLLGHGQPLNWISLEQNGIMVELPQLTIHQMPCKWGWALALTNVI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fucosidase, alpha-L- 2, plasma
别名
计算分子量 54 kDa
观测分子量 54-60 kDa
GenBank蛋白编号BC003060
基因名称 FUCA2
Gene ID (NCBI) 2519
RRIDAB_2104956
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BTY2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Alpha-L-fucosidase 2 (FUCA2) is a gene that codes for a secreted non-lysosomal enzyme and belongs to the glycosyl hydrolase 29 (GH29) family. The enzyme is responsible for the removal of the alpha-1,6-fucose linked to N-acetylglucosamine residue of glycoproteins. Serum FUCA2 is a potential biomarker used for early detection of esophageal squamous cell carcinoma (ESCC) and hepatocellular carcinoma (HCC). FUCA2 has 2 isoforms with the molecular mass of 20 and 54 kDa (PMID: 34935631). Sometimes higher molecular weight around 60 kDa can also be observed, which may be a modified variant of FUCA2.

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