验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, COLO 320 cells |
| Positive IHC detected in | human testis tissue, human liver cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IHC | See 5 publications below |
产品信息
16420-1-AP targets FUCA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag9612 Product name: Recombinant human FUCA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 113-461 aa of BC017338 Sequence: EEWADLFQAAGAKYVVLTTKHHEGFTNWPSPVSWNWNSKDVGPHRDLVGELGTALRKRNIRYGLYHSLLEWFHPLYLLDKKNGFKTQHFVSAKTMPELYDLVNSYKPDLIWSDGEWECPDTYWNSTNFLSWLYNDSPVKDEVVVNDRWGQNCSCHHGGYYNCEDKFKPQSLPDHKWEMCTSIDKFSWGYRRDMALSDVTEESEIISELVQTVSLGGNYLLNIGPTKDGLIVPIFQERLLAVGKWLSINGEAIYASKPWRVQWEKNTTSVWYTSKGSAVYAIFLHWPENGVLNLESPITTSTTKITMLGIQGDLKWSTDPDKGLFISLPQLPPSAVPAEFAWTIKLTGVK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | fucosidase, alpha-L- 1, tissue |
| 别名 | Alpha-L-fucosidase 1, Alpha-L-fucosidase I, Alpha-L-fucoside fucohydrolase 1, EC:3.2.1.51, FUCA |
| 计算分子量 | 461 aa, 53 kDa |
| 观测分子量 | 50-56 kDa |
| GenBank蛋白编号 | BC017338 |
| 基因名称 | FUCA1 |
| Gene ID (NCBI) | 2517 |
| RRID | AB_2294111 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P04066 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FUCA1(a-l-Fucosidase) is a lysosomal glycosidase which is ubiquitous in eukaryotic cells. This enzyme is usually found as a soluble component of the lysosome and functions as an acid hydrolase in the degradation of numerous and diverse fucoglycoconjugates.The mammalian a-l-fucosidases are relatively large, multisubunit glycoproteins which exist in multiple molecular isoforms.This enzyme may have a role in sperm-egg interactions in the reproductive tract of the female rat. There is a precursor 54 kDa form of rat sperm fucosidase which is processed to a mature 52 kDa form of the enzyme(PMID:8836125). This protein from rat epididymis is a tetramer of 210-220 kDa, made up by two pairs of subunits of 47 and 60 kDa, and the one from human seminal plasma exists in different oligomeric forms, that is, as a dimer, as a tetramer, and as a hexamer made up of a single subunit of 56-57 kDa(PMID:16736526).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FUCA1 antibody 16420-1-AP | Download protocol |
| IHC protocol for FUCA1 antibody 16420-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncotarget Reduced expression of α-L-Fucosidase-1 (FUCA-1) predicts recurrence and shorter cancer specific survival in luminal B LN+ breast cancer patients. | ||
Gene Potential predictive value of immune-related genes FUCA1 and NCKAP1L for osteosarcoma metastasis | ||
Cancer Genomics Proteomics High Resolution Proteomic Analysis of the Cervical Cancer Cell Lines Secretome Documents Deregulation of Multiple Proteases. | ||
Pathol Int Alpha-L-fucosidase-1 is a diagnostic marker that distinguishes mucoepidermoid carcinoma from squamous cell carcinoma. | ||
Oncotarget Proteomics analysis of bladder cancer invasion: Targeting EIF3D for therapeutic intervention. |
