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验证数据展示

  • WB analysis of SH-SY5Y using 24864-1-AP (same clone as 24864-1-PBS)

    WB analysis of SH-SY5Y using 24864-1-AP (same clone as 24864-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24864-1-AP (FUBP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24864-1-PBS in a different storage buffer formulation.

  • IP experiment of SH-SY5Y using 24864-1-AP (same clone as 24864-1-PBS)

    IP experiment of SH-SY5Y using 24864-1-AP (same clone as 24864-1-PBS)

    IP result of anti-FUBP1 (IP:24864-1-AP, 4ug; Detection:24864-1-AP 1:3000) with SH-SY5Y cells lysate 1480 ug. This data was developed using the same antibody clone with 24864-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 24864-1-AP (same clone as 24864-1-PBS)

    IF Staining of HeLa using 24864-1-AP (same clone as 24864-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FUBP1 antibody (24864-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 24864-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

FUBP1 Polyclonal antibody, PBS Only

FUBP1 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 24864-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, IP, Indirect ELISA

别名

Far upstream element-binding protein 1, FUSE-binding protein 1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

24864-1-PBS targets FUBP1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag13149

Product name: Recombinant human FUBP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 303-653 aa of BC017247

Sequence: DAGVRIQFKPDDGTTPERIAQITGPPDRCQHAAEIITDLLRSVQAGNPGGPGPGGRGRGRGQGNWNMGPPGGLQEFNFIVPTGKTGLIIGKGGETIKSISQQSGARIELQRNPPPNADPNMKLFTIRGTPQQIDYARQLIEEKIGGPVNPLGPPVPHGPHGVPGPHGPPGPPGPGTPMGPYNPAPYNPGPPGPAPHGPPAPYAPQGWGNAYPHWQQQAPPDPAKAGTDPNSAAWAAYYAHYYQQQAQPPPAAPAGAPTTTQTNGQGDQQNPAPAGQVDYTKAWEEYYKKMGQAVPAPTGAPPGGQPDYSAAWAEYYRQQAAYYAQTSPQGMPQHPPAPQCRFDPASIELAL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 far upstream element (FUSE) binding protein 1
别名 Far upstream element-binding protein 1, FUSE-binding protein 1
计算分子量 653 aa, 69 kDa
观测分子量 69 kDa
GenBank蛋白编号BC017247
基因名称 FUBP1
Gene ID (NCBI) 8880
RRIDAB_2879762
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96AE4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FUBP1 also termed as FBP and FUSE-binding protein 1 is 644 amino-acid protein, which localizes in the nucleus. FUBP1 binds to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter and regulates the MYC expression, indicating that FBP1 functions as a growth-dependent regulator of c-Myc expression. FUBP1 binds to FUSE, and PUF60, and forms a stable tripartite complex, which represses activated but not basal c-myc transcription after transcription initiation by delaying promoter escape. FUBP1 may be acting both as activator and repressor of transcription.

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