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验证数据展示

  • WB analysis using 27226-1-AP (same clone as 27226-1-PBS)

    WB analysis using 27226-1-AP (same clone as 27226-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27226-1-AP (FTO antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27226-1-PBS in a different storage buffer formulation.

  • WB analysis of MDA-MB-231 using 27226-1-AP (same clone as 27226-1-PBS)

    WB analysis of MDA-MB-231 using 27226-1-AP (same clone as 27226-1-PBS)

    WB result of FTO antibody (27226-1-AP; 1:10000; room temperature for 1.5 hours) with wild-type and FTO knockout MDA-MB-231 cells. This data was developed using the same antibody clone with 27226-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 27226-1-AP (same clone as 27226-1-PBS)

    IHC staining of human liver cancer using 27226-1-AP (same clone as 27226-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27226-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FTO antibody included
  • IHC staining of human liver cancer using 27226-1-AP (same clone as 27226-1-PBS)

    IHC staining of human liver cancer using 27226-1-AP (same clone as 27226-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27226-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FTO antibody included
  • IF Staining of human lymphoma using 27226-1-AP (same clone as 27226-1-PBS)

    IF Staining of human lymphoma using 27226-1-AP (same clone as 27226-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using FTO antibody (27226-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27226-1-PBS in a different storage buffer formulation.

  • IF Staining of human lymphoma using 27226-1-AP (same clone as 27226-1-PBS)

    IF Staining of human lymphoma using 27226-1-AP (same clone as 27226-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using FTO antibody (27226-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27226-1-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 27226-1-AP (same clone as 27226-1-PBS)

    FC experiment of SH-SY5Y using 27226-1-AP (same clone as 27226-1-PBS)

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human FTO (27226-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 27226-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

FTO Polyclonal antibody, PBS Only

FTO Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA
Cat No. 27226-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF-P, FC (Intra), Indirect ELISA

别名

KIAA1752

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy FTO Ready-To-Use IHC Kit. FTO primary antibody included.

产品信息

27226-1-PBS targets FTO in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag26095

Product name: Recombinant human FTO protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 400-505 aa of NM_001080432

Sequence: MAQLEALWKKMEGVTNAVLHEVKREGLPVEQRNEILTAILASLTARQNLRREWHARCQSRIARTLPADQKPECRPYWEKDDASMPLPFDLTDIVSELRGQLLEAKP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fat mass and obesity associated
别名 KIAA1752
计算分子量 58 kDa
观测分子量 58 kDa
GenBank蛋白编号NM_001080432
基因名称 FTO
Gene ID (NCBI) 79068
RRIDAB_2880809
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9C0B1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m6A) residues in RNA in vitro. Variants in the FTO (fat mass and obesity associated) gene are associated with increased body mass index in humans.

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