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验证数据展示

  • WB analysis using 81166-3-RR (same clone as 81166-3-PBS)

    WB analysis using 81166-3-RR (same clone as 81166-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81166-3-RR (FTL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81166-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 81166-3-RR (same clone as 81166-3-PBS)

    IHC staining of mouse liver using 81166-3-RR (same clone as 81166-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81166-3-RR (FTL antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81166-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FTL antibody included
  • IF Staining of A549 using 81166-3-RR (same clone as 81166-3-PBS)

    IF Staining of A549 using 81166-3-RR (same clone as 81166-3-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using FTL antibody (81166-3-RR, Clone: 251048G12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81166-3-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 81166-3-RR (same clone as 81166-3-PBS)

    IF Staining of HepG2 using 81166-3-RR (same clone as 81166-3-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FTL antibody (81166-3-RR, Clone: 251048G12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81166-3-PBS in a different storage buffer formulation.

FTL Recombinant monoclonal antibody, PBS Only

FTL Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 81166-3-PBS

产品说明书

CloneNo. 251048G12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Ferritin, Ferritin light chain, Ferritin L subunit

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy FTL Ready-To-Use IHC Kit. FTL primary antibody included.

产品信息

81166-3-PBS targets FTL in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag20637

Product name: Recombinant human FTL protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-175 aa of BC008439

Sequence: MSSQIRQNYSTDVEAAVNSLVNLYLQASYTYLSLGFYFDRDDVALEGVSHFFRELAEEKREGYERLLKMQNQRGGRALFQDIKKPAEDEWGKTPDAMKAAMALEKKLNQALLDLHALGSARTDPHLCDFLETHFLDEEVKLIKKMGDHLTNLHRLGGPEAGLGEYLFERLTLKHD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ferritin, light polypeptide
别名 Ferritin, Ferritin light chain, Ferritin L subunit
计算分子量 19 kDa
观测分子量18 kDa
GenBank蛋白编号BC008439
基因名称 Ferritin light chain
Gene ID (NCBI) 2512
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP02792
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FTL belongs to the ferritin family. It stores iron in a soluble, non-toxic, readily available form. FTL is important for iron homeostasis. It plays a role in delivery of iron to cells. FTL mediates iron uptake in capsule cells of the developing kidney. Mutation of FTL will cause hereditary hyperferritinemia-cataract syndrome (HHCS) or neurodegeneration with brain iron accumulation type 3 (NBIA3). Ferritin light polypeptide (FTL) and ferritin heavy polypeptide (FTH1) were the main constituents the striatum and cerebellar cortex revealed.

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