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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 60875-5-Ig (same clone as 60875-5-PBS)

    WB analysis using 60875-5-Ig (same clone as 60875-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60875-5-Ig (Ferritin heavy chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60875-5-PBS in a different storage buffer formulation.

  • WB analysis using 60875-5-Ig (same clone as 60875-5-PBS)

    WB analysis using 60875-5-Ig (same clone as 60875-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60875-5-Ig (Ferritin heavy chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60875-5-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 60875-5-Ig (same clone as 60875-5-PBS)

    IHC staining of rat liver using 60875-5-Ig (same clone as 60875-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 60875-5-Ig (FTH1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60875-5-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 60875-5-Ig (same clone as 60875-5-PBS)

    IHC staining of rat liver using 60875-5-Ig (same clone as 60875-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 60875-5-Ig (FTH1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60875-5-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 60875-5-Ig (same clone as 60875-5-PBS)

    IF Staining of HepG2 using 60875-5-Ig (same clone as 60875-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Ferritin heavy chain antibody (60875-5-Ig, Clone: 3G8D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60875-5-PBS in a different storage buffer formulation.

Ferritin heavy chain Monoclonal antibody, PBS Only

Ferritin heavy chain Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 60875-5-PBS

产品说明书

CloneNo. 3G8D7

宿主/亚型

Mouse / IgG2a

种属反应性

human, rat, rabbit

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Ferritin, FTH1, Cell proliferation-inducing gene 15 protein, EC:1.16.3.1, Ferritin heavy chain, N-terminally processed

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

60875-5-PBS targets Ferritin heavy chain in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat, rabbit samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, rat, rabbit
免疫原 Ferritin heavy chain fusion protein Ag21386 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ferritin, heavy polypeptide 1
别名 Ferritin, FTH1, Cell proliferation-inducing gene 15 protein, EC:1.16.3.1, Ferritin heavy chain, N-terminally processed
计算分子量 183 aa, 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC000857
基因名称 FTH1
Gene ID (NCBI) 2495
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP02794
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ferritin heavy chain(FTH1, ferritin, heavy polypeptide 1 ), as a 21 kDa subunit of the ferritin complex, exhibits ferroxidase activity, which serves an essential role in catalyzing the conversion of the ferrous ions (Fe2+) to the ferric form (Fe3+). FTH1 regulates angiogenesis during inflammation and malignancy by binding to high molecular weight kininogen (HKa) and blocking its anti-angiogenic activity on endothelial cells in vitro and in vivo(PMID: 33260500). FTH1 has been shown to protect proximal tube epithelial cells and kidneys against the activity of free iron in reactive oxygen species generation. FTH1 plays an important role in the maintenance of the cellular iron balance in ferroptosis.