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验证数据展示

  • WB analysis using 83664-2-RR

    WB analysis using 83664-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83664-2-RR (FTCD antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 83664-2-RR

    FC experiment of HepG2 using 83664-2-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug FTCD Recombinant antibody (83664-2-RR, Clone:240654G9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83664-2-RR

    Affinity and Kinetic Characterization of 83664-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83664-2-RR against Human FTCD were performed. The affinity constant is 6.37 nM.

FTCD Recombinant antibody

FTCD Recombinant Antibody for WB, ELISA
Cat No. 83664-2-RR

产品说明书

CloneNo. 240654G9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

Glutamate formiminotransferase, Glutamate formimidoyltransferase, Formiminotransferase-cyclodeaminase, Formimidoyltransferase-cyclodeaminase, Formimidoyltetrahydrofolate cyclodeaminase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue, human kidney tissue
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy FTCD Ready-To-Use IHC Kit. FTCD primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83664-2-RR targets FTCD in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 FTCD fusion protein Ag16646 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 formiminotransferase cyclodeaminase
别名 Glutamate formiminotransferase, Glutamate formimidoyltransferase, Formiminotransferase-cyclodeaminase, Formimidoyltransferase-cyclodeaminase, Formimidoyltetrahydrofolate cyclodeaminase
计算分子量 541 aa, 59 kDa
观测分子量58 kDa
GenBank蛋白编号BC136395
基因名称 FTCD
Gene ID (NCBI) 10841
RRIDAB_3671271
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95954
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FTCD (formimidoyltransferase cyclodeaminase) is critical for the catabolism of histidine, a . This process is one-carbon units that can enter the one-carbon/folate cycle, which provides methyl groups for arsenic metabolism (PMID: 30893314). In addition, FTCD could serve as a predictive and prognostic marker for patients with HCC (PMID: 37675273).

实验方案

Product Specific Protocols
WB protocol for FTCD antibody 83664-2-RRDownload protocol
FC protocol for FTCD antibody 83664-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols