- Featured Product
- KD/KO Validated
FSHR Polyclonal antibody
FSHR Polyclonal Antibody for FC, IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (5)
Applications
FC, IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse ovary tissue, rat ovary tissue, DU 145 cells, PC-3 cells, HeLa cells |
| Positive IHC detected in | mouse ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells, HepG2 cells |
| Positive FC detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22665-1-AP targets FSHR in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC, IF/ICC, IHC, WB, ELISA |
| Cited Applications | WB, IF, FC, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, goat, chicken, Sheep, geese, bovine |
| Immunogen | FSHR fusion protein Ag18158 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | follicle stimulating hormone receptor |
| Synonyms | Follitropin receptor, FSH R, FSHR, FSHRO, LGR1, ODG1 |
| Calculated Molecular Weight | 695 aa, 78 kDa |
| Observed Molecular Weight | 71-78 kDa |
| GenBank Accession Number | BC118548 |
| Gene Symbol | FSHR |
| Gene ID (NCBI) | 2492 |
| RRID | AB_2631204 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P23945 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FSHR antibody 22665-1-AP | Download protocol |
| IHC protocol for FSHR antibody 22665-1-AP | Download protocol |
| IF protocol for FSHR antibody 22665-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Res Blocking FSH inhibits hepatic cholesterol biosynthesis and reduces serum cholesterol. | ||
Autophagy Autophagy regulates differentiation of ovarian granulosa cells through degradation of WT1 | ||
Redox Biol Novel ovarian endometriosis model causes infertility via iron-mediated oxidative stress in mice. | ||
ACS Appl Mater Interfaces A Novel Three-Dimensional Follicle Culture System Decreases Oxidative Stress and Promotes the Prolonged Culture of Human Granulosa Cells | ||
Free Radic Biol Med Iron overload modulates follicular microenvironment via ROS/HIF-1α/FSHR signaling | ||
Ecotoxicol Environ Saf Chlorogenic acid rescues zearalenone induced injury to mouse ovarian granulosa cells. |
