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FSD2 Polyclonal antibody

FSD2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 25609-1-AP

Print datasheet

Synonyms

FSD2, RP11 127F21, SPRYD1

验证数据展示

  • WB analysis of mouse heart using 25609-1-AP

    WB analysis of mouse heart using 25609-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 25609-1-AP (FSD2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • WB analysis using 25609-1-AP

    WB analysis using 25609-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25609-1-AP (FSD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 25609-1-AP

    IHC staining of mouse heart using 25609-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25609-1-AP (FSD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with .

  • IHC staining of mouse heart using 25609-1-AP

    IHC staining of mouse heart using 25609-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25609-1-AP (FSD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with .

经过测试的应用

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25609-1-AP targets FSD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen FSD2 fusion protein Ag22229 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fibronectin type III and SPRY domain containing 2
Synonyms FSD2, RP11 127F21, SPRYD1
Calculated Molecular Weight 749 aa, 85 kDa
Observed Molecular Weight 95-105 kDa
GenBank Accession NumberBC130569
Gene Symbol FSD2
Gene ID (NCBI) 123722
RRIDAB_2880159
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA1L4K1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FSD2 antibody 25609-1-APDownload protocol
IHC protocol for FSD2 antibody 25609-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols