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验证数据展示

  • WB analysis of PC-3 using 13536-1-AP

    WB analysis of PC-3 using 13536-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 13536-1-AP (FSCN3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 13536-1-AP

    IHC staining of human prostate cancer using 13536-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13536-1-AP (FSCN3 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human prostate cancer using 13536-1-AP

    IHC staining of human prostate cancer using 13536-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13536-1-AP (FSCN3 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of PC-3 using 13536-1-AP

    IF Staining of PC-3 using 13536-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 13536-1-AP (FSCN3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of PC-3 using 13536-1-AP

    IF Staining of PC-3 using 13536-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using FSCN3 antibody (13536-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

FSCN3 Polyclonal antibody

FSCN3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13536-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Testis fascin, Fascin-3, Fascin 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13536-1-AP targets FSCN3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 FSCN3 fusion protein Ag4459 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fascin homolog 3, actin-bundling protein, testicular (Strongylocentrotus purpuratus)
别名 Testis fascin, Fascin-3, Fascin 3
计算分子量 498 aa, 57 kDa
观测分子量 57 kDa
GenBank蛋白编号BC035606
基因名称 FSCN3
Gene ID (NCBI) 29999
RRIDAB_2106517
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQT6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FSCN3 antibody 13536-1-APDownload protocol
IHC protocol for FSCN3 antibody 13536-1-APDownload protocol
IF protocol for FSCN3 antibody 13536-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols