验证数据展示
经过测试的应用
| Positive WB detected in | DU 145 cells, HEK-293 cells, HeLa cells, U2OS cells, NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24085-1-AP targets FRYL in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag21333 Product name: Recombinant human FRYL protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 2605-2654 aa of BC021803 Sequence: MPEPLAPESYPESVCEEDVTLALKELDERCEEEEADFSGLSSQDEEEQDG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | FRY-like |
| 别名 | Protein furry homolog like, KIAA0826, FRY like, AF4P12 |
| 计算分子量 | 3013 aa, 340 kDa |
| 观测分子量 | 340 kDa |
| GenBank蛋白编号 | BC021803 |
| 基因名称 | FRYL |
| Gene ID (NCBI) | 285527 |
| RRID | AB_3742103 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | O94915 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FRYL, or FRY-like transcription coactivator, is a protein that belongs to the Furry protein family, which is evolutionarily conserved from yeast to humans. FRYL has almost identical functional domains to the well-characterized Fry protein, which is involved in many processes, including cell polarization, regulation of the actin cytoskeleton, and patterning of sensory neuron dendritic fields (PMID: 34386489). FRYL is a prognostic marker in certain cancers, including Kidney renal clear cell carcinoma, Ovary serous cystadenocarcinoma, and Rectum adenocarcinoma, suggesting its potential role in cancer progression.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FRYL antibody 24085-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
