• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

FRS3 Polyclonal antibody

FRS3 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 12249-1-AP

Print datasheet

Synonyms

FGFR signaling adaptor SNT2, FGFR substrate 3, FRS2B, FRS2beta, FRS3, SNT 2, SNT2

验证数据展示

  • IHC staining of human skin using 12249-1-AP

    IHC staining of human skin using 12249-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 12249-1-AP (FRS3 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human skin using 12249-1-AP

    IHC staining of human skin using 12249-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 12249-1-AP (FRS3 antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis of human placenta using 12249-1-AP

    WB analysis of human placenta using 12249-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 12249-1-AP (FRS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-3 using 12249-1-AP

    IF Staining of PC-3 using 12249-1-AP

    Immunofluorescent analysis of PC-3 cells using 12249-1-AP (FRS3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12249-1-AP targets FRS3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen FRS3 fusion protein Ag2896 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fibroblast growth factor receptor substrate 3
Synonyms FGFR signaling adaptor SNT2, FGFR substrate 3, FRS2B, FRS2beta, FRS3, SNT 2, SNT2
Calculated Molecular Weight 492 aa, 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC010611
Gene Symbol FRS3
Gene ID (NCBI) 10817
RRIDAB_2106244
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43559
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FRS3 antibody 12249-1-APDownload protocol
IHC protocol for FRS3 antibody 12249-1-APDownload protocol
IF protocol for FRS3 antibody 12249-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols