验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, SK-N-SH cells, Neuro-2a cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25033-1-AP targets FRMD7 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag18161 Product name: Recombinant human FRMD7 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 385-714 aa of BC114371 Sequence: LEVTFATELEHSKPEADPTLLHQSQSSSSFPFIYMDPVFNTEPNPNPDPRDIFSERSSLSSFQTSCKFSGNHMSIYSGLTSKVRPAKQLTYTDVPYIPCTGQQVGIMPPQVFFYVDKPPQVPRWSPIRAEERTSPHSYVEPTAMKPAERSPRNIRMKSFQQDLQVLQEAIARTSGRSNINVGLEEEDPNLEDAFVCNIQEQTPKRSQSQSDMKTIRFPFGSEFRPLGPCPALSHKADLFTDMFAEQELPAVLMDQSTAERYVASESSDSESEILKPDYYALYGKEIRSPMARIRLSSGSLQLDEEDEDAYFNTPTAEDRTSLKPCNYFLA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | FERM domain containing 7 |
| 别名 | FERM domain containing 7, NYS, NYS1 |
| 计算分子量 | 714 aa, 82 kDa |
| 观测分子量 | 80-90 kDa |
| GenBank蛋白编号 | BC114371 |
| 基因名称 | FRMD7 |
| Gene ID (NCBI) | 90167 |
| RRID | AB_3742133 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6ZUT3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The 714-amino acid FRMD7 protein contains an N-terminal FERM domain and FERM-adjacent (FA) domain. FRMD7 shares close homology with FARP1 (FERM, RhoGEF, and pleckstrin domain protein 1) as well as FARP2. FRMD7 also promotes neurite elongation at the actin-rich growth cone ends through the modulation of the actin cytoskeleton.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FRMD7 antibody 25033-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
