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验证数据展示

  • WB analysis using 16197-1-AP (same clone as 16197-1-PBS)

    WB analysis using 16197-1-AP (same clone as 16197-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16197-1-AP (FRK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16197-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 16197-1-AP (same clone as 16197-1-PBS)

    IF Staining of HepG2 using 16197-1-AP (same clone as 16197-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using FRK antibody 16197-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 16197-1-PBS in a different storage buffer formulation.

FRK Polyclonal antibody, PBS Only

FRK Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 16197-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

16197-1-PBS targets FRK in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10372

Product name: Recombinant human FRK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 235-505 aa of BC012916

Sequence: QLLKRLGSGQFGEVWEGLWNNTTPVAVKTLKPGSMDPNDFLREAQIMKNLRHPKLIQLYAVCTLEDPIYIITELMRHGSLQEYLQNDTGSKIHLTQQVDMAAQVASGMAYLESRNYIHRDLAARNVLVGEHNIYKVADFGLARVFKVDNEDIYESRHEIKLPVKWTAPEAIRSNKFSIKSDVWSFGILLYEIITYGKMPYSGMTGAQVIQMLAQNYRLPQPSNCPQQFYNIMLECWNAEPKERPTFETLRWKLEDYFETDSSYSDANNFIR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fyn-related kinase
别名
计算分子量 505 aa, 56 kDa
观测分子量 54-57 kDa
GenBank蛋白编号BC012916
基因名称 FRK
Gene ID (NCBI) 2444
RRIDAB_10639525
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP42685
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The fyn-related kinase (FRK) belongs to the tyrosine kinase family of protein kinases and is also named PTK5 (protein-tyrosine kinase 5), RAK(Nuclear tyrosine protein kinase). The Frk is a tyrosine kinase expressed in various tissues, such as the gastrointestinal tract and pancreatic islet cells. It affects pancreatic beta cell number during embryogenesis and promotes beta cell cytotoxic signals in response to streptozotocin(PMID:20157399). It may function as a tumor suppressor(PMID:19345329).

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