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验证数据展示

  • WB analysis of mouse colon using 24937-1-AP

    WB analysis of mouse colon using 24937-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 24937-1-AP (FPGT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 24937-1-AP

    IHC staining of human colon using 24937-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24937-1-AP (FPGT Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon using 24937-1-AP

    IHC staining of human colon using 24937-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24937-1-AP (FPGT Antibody) at dilution of 1:50 (under 40x lens).

FPGT Polyclonal antibody

FPGT Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24937-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

FPGT, GDP L fucose diphosphorylase, GDP L fucose pyrophosphorylase, GFPP

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse colon tissue
Positive IHC detected inhuman colon tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24937-1-AP targets FPGT in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag20655

Product name: Recombinant human FPGT protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-354 aa of BC032308

Sequence: MAAARDPPEVSLREATQRKLRRFSELRGKLVARGEFWDIVAITAADEKQELAYNQQLSEKLKRKELPLGVQYHVFVDPAGAKIGNGGSTLCALQCLEKLYGDKWNSFTILLIHSGGYSQRLPNASALGKIFTALPLGNPIYQMLELKLAMYIDFPLNMNPGILVTCADDIELYSIGEFEFIRFDKPGFTALAHPSSLTIGTTHGVFVLDPFDDLKHRDLEYRSCHRFLHKPSIEKMYQFNAVCRPGNFCQQDFAGGDIADLKLDSDYVYTDSLFYMDHKSAKMLLAFYEKIGTLSCEIDAYGDFLQALGPGATVEYTRNTSNVIKEESELVEMRQRIFHLLKGTSLNVVVLNNS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fucose-1-phosphate guanylyltransferase
别名 FPGT, GDP L fucose diphosphorylase, GDP L fucose pyrophosphorylase, GFPP
计算分子量 67 kDa
观测分子量 38 kDa
GenBank蛋白编号BC032308
基因名称 FPGT
Gene ID (NCBI) 8790
RRIDAB_2879808
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14772
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FPGT(Fucose-1-phosphate guanylyltransferase) is also named as GFPP. This protein catalyzes the formation of GDP-L-fucose from GTP and L-fucose-1-phosphate. It also functions as a salvage pathway to reutilize L-fucose arising from the turnover of glycoproteins and glycolipids. FPGT has 6 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IHC protocol for FPGT antibody 24937-1-APDownload protocol
WB protocol for FPGT antibody 24937-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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