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验证数据展示

  • WB analysis using 27616-1-AP

    WB analysis using 27616-1-AP

    HeLa mitochondrial extracts were subjected to SDS PAGE followed by western blot with 27616-1-AP (FPGS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 27616-1-AP

    IHC staining of mouse kidney using 27616-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27616-1-AP (FPGS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FPGS Polyclonal antibody

FPGS Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27616-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Tetrahydrofolate synthase

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa mitochondrial extract
Positive IHC detected inmouse kidney tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27616-1-AP targets FPGS in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag24775

Product name: Recombinant human FPGS protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 397-587 aa of BC064393

Sequence: QGRERPSGGPEVRVLLFNATGDRDPAALLKLLQPCQFDYAVFCPNLTEVSSTGNADQQNFTVTLDQVLLRCLEHQQHWNHLDEEQASPDLWSVPSPEPGGSASLLLAPHPPHTCSASSLVFSCISHALQWISQGRDPIFQPPSPPKGLLTHPVAHSGASILREAAAIHVLVTGSLHLVGGVLKLLEPALSQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 folylpolyglutamate synthase
别名 Tetrahydrofolate synthase
计算分子量 65 kDa
观测分子量60-65 kDa
GenBank蛋白编号BC064393
基因名称 FPGS
Gene ID (NCBI) 2356
RRIDAB_3669615
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ05932
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FPGS is located in the cytosol and mitochondria of mammalian tissues, with current evidence supporting a single gene encoding for both the cytosolic and mitochondrial isoenzymes. FPGS catalyzes a rate-limiting polyglutamylation step in the folic acid metabolic pathway and increases the intracellular retention of folates (PMID: 8907263, PMID: 31611592). FPGS has 4 isoforms with the molecular mass of 59-65 kDa.

实验方案

Product Specific Protocols
IHC protocol for FPGS antibody 27616-1-APDownload protocol
WB protocol for FPGS antibody 27616-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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