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验证数据展示

  • IHC staining of human tonsillitis using 68803-2-Ig

    IHC staining of human tonsillitis using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FOXP3 antibody included
  • WB analysis of MJ using 68803-2-Ig

    WB analysis of MJ using 68803-2-Ig

    MJ cells were subjected to SDS PAGE followed by western blot with 68803-2-Ig (FOXP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 68803-2-Ig

    IHC staining of human tonsillitis using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FOXP3 antibody included
  • IHC staining of human rectal cancer using 68803-2-Ig

    IHC staining of human rectal cancer using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FOXP3 antibody included
  • IHC staining of human appendicitis using 68803-2-Ig

    IHC staining of human appendicitis using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FOXP3 antibody included

FOXP3 Monoclonal antibody

FOXP3 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68803-2-Ig

产品说明书

CloneNo. 2A1E7

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, ELISA

别名

2A1E7, AIID, DIETER, forkhead box P3, Forkhead box protein P3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMJ cells
Positive IHC detected inhuman tonsillitis tissue, human appendicitis tissue, human lymphoma tissue, human ovary cancer tissue, human rectal cancer tissue, MJ cells
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy FOXP3 Ready-To-Use IHC Kit. FOXP3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68803-2-Ig targets FOXP3 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag23089

Product name: Recombinant human FOXP3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 107-196 aa of BC113401

Sequence: STVDAHARTPVLQVHPLESPAMISLTPPTTATGVFSLKARPGLPPGINVASLEWVSREPALLCTFPNPSAPRKDSTLSAVPQSSYPLLAN

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 forkhead box P3
别名 2A1E7, AIID, DIETER, forkhead box P3, Forkhead box protein P3
计算分子量 431 aa, 47 kDa
观测分子量47 kDa
GenBank蛋白编号BC113401
基因名称 FOXP3
Gene ID (NCBI) 50943
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9BZS1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FOXP3 is a transcription factor belonging to the Forkhead box (FOX) protein family. It contains a forkhead-winged helix domain that acts as a transcriptional activator or repressor. FOXP3 is the master regulator of regulatory T cells (Tregs), a subset of CD4+ T cells with potent suppressive activity. It plays a critical role in maintaining self-tolerance and preventing autoimmune diseases by controlling the development and function of Tregs. The expression of FOXP3 is quite limited, and the cellular taxa present in the tissue are relatively low. Therefore, if you intend to perform Western blotting (WB), it is advisable to enrich the target cells prior to detection.

实验方案

Product Specific Protocols
WB protocol for FOXP3 antibody 68803-2-IgDownload protocol
IHC protocol for FOXP3 antibody 68803-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols