FOXP3 Polyclonal antibody

FOXP3 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IP, WB,ELISA and More (3)

Conjugate

Unconjugated

Cat No : 22228-1-AP

Print datasheet

Synonyms

AIID, DIETER, forkhead box P3, Forkhead box protein P3, FOXP3, IPEX, JM2, PIDX, Scurfin, XPID



经过测试的应用

Positive WB detected inHeLa cells, multi-cells, multi-cells/tissue, A375 cells, mouse brain tissue, HEK-293 cells
Positive IP detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 22228-1-AP is FOXP3 Fusion Protein expressed in E. coli.

Tested Applications IP, WB,ELISA
Cited ApplicationsWB, FC, CoIP, ChIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Immunogen FOXP3 fusion protein Ag17571 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name forkhead box P3
Synonyms AIID, DIETER, forkhead box P3, Forkhead box protein P3, FOXP3, IPEX, JM2, PIDX, Scurfin, XPID
Calculated Molecular Weight 431 aa, 47 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC113401
Gene Symbol FOXP3
Gene ID (NCBI) 50943
RRIDAB_11182376
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZS1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FOXP3, also named as IPEX, JM2 and Scurfin, is a transcription factor. POXP3 plays a critical role in the control of immune response. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human FOXP3.

实验方案

Product Specific Protocols
WB protocol for FOXP3 antibody 22228-1-APDownload protocol
IP protocol for FOXP3 antibody 22228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse

Acta Pharm Sin B

Self-assembled FeS-based cascade bioreactor with enhanced tumor penetration and synergistic treatments to trigger robust cancer immunotherapy

Authors - Hao Ren
mouse

Cancer Res

Epigenetic regulation of CXCL12 plays a critical role in mediating tumor progression and the immune response in osteosarcoma.

Authors - Binghao Li
mouse

Biomaterials

LncRNA-targeting bio-scaffold mediates triple immune effects for postoperative colorectal cancer immunotherapy.

Authors - Feng Liu
humanWB

Clin Transl Med

MiRNA-516a promotes bladder cancer metastasis by inhibiting MMP9 protein degradation via the AKT/FOXO3A/SMURF1 axis.

Authors - Yuanyuan Chang
humanWB

J Autoimmun

NF-κB-driven miR-34a impairs Treg/Th17 balance via targeting Foxp3.

Authors - Mengxiao Xie
ratWB

Biomed Pharmacother

Kuijieling regulates the differentiation of Treg and Th17 cells to ameliorate experimental colitis in rats.

Authors - Yu Long