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FOXP1 Polyclonal antibody

FOXP1 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 22051-1-AP

Print datasheet

Synonyms

12CC4, forkhead box P1, Forkhead box protein P1, FOXP1, hFKH1B, HSPC215, QRF1

验证数据展示

  • WB analysis of Jurkat using 22051-1-AP

    WB analysis of Jurkat using 22051-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse embryo using 22051-1-AP

    IHC staining of mouse embryo using 22051-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 22051-1-AP (FOXP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse embryo using 22051-1-AP

    IHC staining of mouse embryo using 22051-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 22051-1-AP (FOXP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of Jurkat using 22051-1-AP

    FC experiment of Jurkat using 22051-1-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human FOXP1 (22051-1-AP) and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inJurkat cells, A549 cells, LNCaP cells, MCF-7 cells, MDA-MB-453s cells, mouse heart tissue, mouse lung tissue, mouse testis tissue, PC-3 cells, Raji cells, Y79 cells
Positive IHC detected inmouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

22051-1-AP targets FOXP1 in WB, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen FOXP1 fusion protein Ag17045 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name forkhead box P1
Synonyms 12CC4, forkhead box P1, Forkhead box protein P1, FOXP1, hFKH1B, HSPC215, QRF1
Calculated Molecular Weight 677 aa, 75 kDa
Observed Molecular Weight 50 kDa, 60-65 kDa, 85 kDa
GenBank Accession NumberBC131720
Gene Symbol FOXP1
Gene ID (NCBI) 27086
RRIDAB_2878980
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H334
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FOXP1, also known as Mac-1-regulated forkhead, is a 677 amino acid protein, which forms homodimers and heterodimers with FOXP2 and FOXP4 (PubMed:25027557). Dimerization is required for DNA-binding. FOXP1 has an important function in neuronal development.9 Mutations of its gene, FOXP1, located on chromosome 3p14.1,7 can result in the development of autism spectrum disorder, intellectual disability, speech and language deficits as well as motor development delay. FOXP1 is also engaged in lung and esophagus morphogenesis, as well as in B-cell development.7,10 The widely researched role of FOXP1 in carcinogenesis is of great importance, although still unclear to some extent. FOXP1 exists some isoforms with MV 75-77 kDa, 65-67 kDa, 12 kDa.

实验方案

Product Specific Protocols
WB protocol for FOXP1 antibody 22051-1-APDownload protocol
IHC protocol for FOXP1 antibody 22051-1-APDownload protocol
FC protocol for FOXP1 antibody 22051-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Mol Med

LINC01116 promotes proliferation and migration of endometrial stromal cells by targeting FOXP1 via sponging miR-9-5p in endometriosis.

Authors - Liangyi Cui
View Article