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验证数据展示

  • WB analysis using 83926-1-RR

    WB analysis using 83926-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83926-1-RR (FOXO6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 83926-1-RR

    IF Staining of A549 using 83926-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using FOXO6 antibody (83926-1-RR, Clone: 240823D10 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IHC staining of rat brain using 83926-1-RR

    IHC staining of rat brain using 83926-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83926-1-RR (FOXO6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat brain using 83926-1-RR

    IF Staining of rat brain using 83926-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using FOXO6 antibody (83926-1-RR, Clone: 240823D10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FOXO6 Recombinant antibody

FOXO6 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 83926-1-RR

产品说明书

CloneNo. 240823D10

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF/ICC, IF-P, ELISA

别名

forkhead box protein O6, 240823D10

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83926-1-RR targets FOXO6 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 forkhead box protein O6
别名 forkhead box protein O6, 240823D10
计算分子量 51 kDa
观测分子量75 kDa
GenBank蛋白编号AI361654
基因名称 FOXO6
Gene ID (NCBI) 100132074
RRIDAB_3671506
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FOXO6 is a member of the murine forkhead family of transcription factors. These forkhead transcription factors may play a role in maintenance. FOXO6 mRNA is predominantly expressed in the developing brain, indicating that FOXO6 may play an important role in the nervous system.

实验方案

Product Specific Protocols
WB protocol for FOXO6 antibody 83926-1-RRDownload protocol
IHC protocol for FOXO6 antibody 83926-1-RRDownload protocol
IF protocol for FOXO6 antibody 83926-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols