验证数据展示
经过测试的应用
| Positive WB detected in | human heart tissue, mouse heart tissue |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy FOXO4 Ready-To-Use IHC Kit. FOXO4 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
19121-1-AP targets FOXO4 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | forkhead box O4 |
| 别名 | AFX, AFX1, forkhead box O4, Forkhead box protein O4, FOXO4, MLLT7 |
| 计算分子量 | 54 kDa |
| 观测分子量 | 45-48 kDa |
| GenBank蛋白编号 | NM_005938 |
| 基因名称 | FOXO4 |
| Gene ID (NCBI) | 4303 |
| RRID | AB_2106957 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P98177 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FOXO4, also named as AFX, AFX1 and MLLT7, is a transcription factor which involved in the regulation of the ins signaling pathway. It binds to ins-response elements (IREs) and can activate transcription of IGFBP1.FOXO down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is involved in negative regulation of the cell cycle. A chromosomal aberration involving FOXO4 is found in acute leukemias. The antibody can recognize isoform 1 and isoform Zeta of FOXO4.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for FOXO4 antibody 19121-1-AP | Download protocol |
| WB protocol for FOXO4 antibody 19121-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
